Aboalfazl Jafari-Sales1 
, Zahra Ghahremani2 , Aylin Golestani2 , Mohadeseh Ghorbanpour Koulani Jadid2 , Kosar Hosseini-Karkaj2 , Kosar Soleymanpour2 , Mohammad Mahdi Salek Faramarzi2 , Mehrdad Pashazadeh3
, Zahra Ghahremani2 , Aylin Golestani2 , Mohadeseh Ghorbanpour Koulani Jadid2 , Kosar Hosseini-Karkaj2 , Kosar Soleymanpour2 , Mohammad Mahdi Salek Faramarzi2 , Mehrdad Pashazadeh3
1- Department of Microbiology, Kaz.C., Islamic Azad University, Kazerun, Iran; Infectious Diseases Research Center, TaMS.C., Islamic Azad University, Tabriz, Iran
2- Infectious Diseases Research Center, TaMS.C., Islamic Azad University, Tabriz, Iran; Department of Cellular and Molecular Biology, Ta.C., Islamic Azad University, Tabriz, Iran
3- Infectious Diseases Research Center, TaMS.C., Islamic Azad University, Tabriz, Iran; Department of Laboratory Sciences and Microbiology, TaMS.C., Islamic Azad University, Tabriz, Iran ,mehrdadpashazadeh85@gmail.com
2- Infectious Diseases Research Center, TaMS.C., Islamic Azad University, Tabriz, Iran; Department of Cellular and Molecular Biology, Ta.C., Islamic Azad University, Tabriz, Iran
3- Infectious Diseases Research Center, TaMS.C., Islamic Azad University, Tabriz, Iran; Department of Laboratory Sciences and Microbiology, TaMS.C., Islamic Azad University, Tabriz, Iran ,
Abstract: (162 Views)
Background: A major contributor to hospital-acquired infections, particularly in burn units, is Pseudomonas aeruginosa (P. aeruginosa). Because this bacterium produces extended-spectrum β-lactamases (ESBLs), antibiotic resistance is a significant treatment concern. In this work, P. aeruginosa isolates from burn victims in Tabriz were examined for antibiotic resistance patterns and the presence of the blaTEM gene.
Methods: In this descriptive-cross-sectional study, 100 clinical isolates of P. aeruginosa were collected from patients hospitalized in the burn wards of Tabriz hospitals over a six-month period. Standard biochemical methods were used to identify microorganisms. Antibiotic resistance patterns were assessed by the disk diffusion technique according to clinical and laboratory standards institute protocols. Additionally, the presence of the blaTEM gene was investigated by polymerase chain reaction, and ESBL production was confirmed by the combined disk test.
Results: The highest resistance rates were observed for levofloxacin (97%) and meropenem (92%), while the lowest was for ceftazidime (69%). Furthermore, 58% (58/100) of the isolates were ESBL-positive, half of which (50%, 29/58) carried the blaTEM gene.
Conclusion: The results of this study indicated that P. aeruginosa strains in burn units of Tabriz hospitals exhibited high antibiotic resistance. Half of ESBL-positive isolates carried the blaTEM gene, highlighting the need for continuous monitoring of antibiotic resistance patterns and prudent use of antibiotics.
Methods: In this descriptive-cross-sectional study, 100 clinical isolates of P. aeruginosa were collected from patients hospitalized in the burn wards of Tabriz hospitals over a six-month period. Standard biochemical methods were used to identify microorganisms. Antibiotic resistance patterns were assessed by the disk diffusion technique according to clinical and laboratory standards institute protocols. Additionally, the presence of the blaTEM gene was investigated by polymerase chain reaction, and ESBL production was confirmed by the combined disk test.
Results: The highest resistance rates were observed for levofloxacin (97%) and meropenem (92%), while the lowest was for ceftazidime (69%). Furthermore, 58% (58/100) of the isolates were ESBL-positive, half of which (50%, 29/58) carried the blaTEM gene.
Conclusion: The results of this study indicated that P. aeruginosa strains in burn units of Tabriz hospitals exhibited high antibiotic resistance. Half of ESBL-positive isolates carried the blaTEM gene, highlighting the need for continuous monitoring of antibiotic resistance patterns and prudent use of antibiotics.
Type of Article: Original article |
Subject:
Basic Medical Sciences
Received: 2025/07/10 | Accepted: 2025/09/28
Received: 2025/07/10 | Accepted: 2025/09/28
References
1. Jafari-Sales A, Khaneshpour H. Molecular Study of BlaIMP and BlaVIM Genes in Pseudomonas Aeruginosa Strains, Producer of Metallo Beta Lactamases Isolated from Clinical Samples in Hospitals and Medical Centers of Tabriz. Paramedical Sciences and Military Health. 2020;14(4):18-25. [View at Publisher] [Google Scholar]
2. Reynolds D, Kollef M. The epidemiology and pathogenesis and treatment of Pseudomonas aeruginosa infections: an update. Drugs. 2021;81(18):2117-31. [View at Publisher] [DOI] [PMID] [Google Scholar]
3. Gong Y, Peng Y, Luo X, Zhang C, Shi Y, Zhang Y, et al. Different infection profiles and antimicrobial resistance patterns between burn ICU and common wards. Front Cell Infect Microbiol. 2021;11:681731. [View at Publisher] [DOI] [PMID] [Google Scholar]
4. Church D, Elsayed S, Reid O, Winston B, Lindsay R. Burn wound infections. Clin Microbiol Rev. 2006;19(2):403-34. [View at Publisher] [DOI] [PMID] [Google Scholar]
5. Abdi FA, Motumma AN, Kalayu AA, Abegaz WE. Prevalence and antimicrobial-resistant patterns of Pseudomonas aeruginosa among burn patients attending Yekatit 12 Hospital Medical College in Addis Ababa, Ethiopia. PloS one. 2024;19(3):e0289586. [View at Publisher] [DOI] [PMID] [Google Scholar]
6. Sah RSP, Dhungel B, Yadav BK, Adhikari N, Thapa Shrestha U, Lekhak B, et al. Detection of TEM and CTX-M Genes in Escherichia coli isolated from clinical specimens at tertiary care heart hospital, Kathmandu, Nepal. Diseases. 2021;9(1):15. [View at Publisher] [DOI] [PMID] [Google Scholar]
7. Mroczkowska JE, Barlow M. Fitness trade-offs in bla TEM evolution. Antimicrob Agents Chemother. 2008;52(7):2340-5. [View at Publisher] [DOI] [PMID] [Google Scholar]
8. Shalmashi H, Farajnia S, Sadeghi M, Tanoumand A, Veissi K, Hamishekar H, et al. Detection of ESBLs types blaCTX-M, blaSHV and blaTEM resistance genes among clinical isolates of Pseudomonas aeruginosa. Gene Rep. 2022;28:101637. [View at Publisher] [DOI] [Google Scholar]
9. Bush K, Jacoby GA. Updated functional classification of β-lactamases. Antimicrob Agents Chemother. 2010;54(3):969-76. [View at Publisher] [DOI] [PMID] [Google Scholar]
10. Bush K, Bradford PA. Epidemiology of β-lactamase-producing pathogens. Clin Microbiol Rev. 2020;33(2):10.1128/cmr. 00047-19. [View at Publisher] [DOI] [PMID] [Google Scholar]
11. Tchakal-Mesbahi A, Metref M, Singh VK, Almpani M, Rahme LG. Characterization of antibiotic resistance profiles in Pseudomonas aeruginosa isolates from burn patients. Burns. 2021;47(8):1833-43. [View at Publisher] [DOI] [PMID] [Google Scholar]
12. Espinal MA, Laszlo A, Simonsen L, Boulahbal F, Kim SJ, Reniero A, et al. Global trends in resistance to antituberculosis drugs. World Health Organization-International :union: against Tuberculosis and Lung Disease Working Group on Anti-Tuberculosis Drug Resistance Surveillance. N Engl J Med. 2001;344(17):1294-303. [View at Publisher] [DOI] [PMID] [Google Scholar]
13. Rahimzadeh Torabi L, Doudi M, Golshani Z. The frequency of blaIMP and blaVIM carbapenemase genes in clinical isolates of Pseudomonas aeruginosa in Isfahan medical centers. medical journal of mashhad university of medical sciences. 2016;59(3):139-47. [View at Publisher] [DOI] [Google Scholar]
14. Drawz SM, Bonomo RA. Three decades of β-lactamase inhibitors. Clin Microbiol Rev. 2010;23(1):160-201. [View at Publisher] [DOI] [PMID] [Google Scholar]
15. Empel J, Filczak K, Mrówka A, Hryniewicz W, Livermore DM, Gniadkowski M. Outbreak of Pseudomonas aeruginosa infections with PER-1 extended-spectrum β-lactamase in Warsaw, Poland: further evidence for an international clonal complex. J Clin Microbiol. 2007;45(9):2829-34. [View at Publisher] [DOI] [PMID] [Google Scholar]
16. Lin S-P, Liu M-F, Lin C-F, Shi Z-Y. Phenotypic detection and polymerase chain reaction screening of extended-spectrum β-lactamases produced by Pseudomonas aeruginosa isolates. J Microbiol Immunol Infect. 2012;45(3):200-7. [View at Publisher] [DOI] [PMID] [Google Scholar]
17. Shams E, Nateghi B, Eshaghiyan A, Behshood P. TEM gene Detection in Clinical Pseudomonas aeruginosa and Escherichia coli Samples. Research in Molecular Medicine. 2019;7(1):35-41. [View at Publisher] [DOI] [Google Scholar]
18. Jafarı-sales A, Mehdizadeh F, Fallah G, Pashazadeh M, Baghi HB. Examining the frequency of carbapenemase genes blaKPC, blaIMP, blaOXA-48, blaSPM, blaNDM, blaVIM, blaGES, blaBIC, blaAIM, blaGIM, blaSIM, and blaDIM in Pseudomonas aeruginosa strains isolated from patients hospitalized in northwest Iran hospitals. Journal of Experimental and Clinical Medicine. 2024;41(3):466-73. [View at Publisher] [Google Scholar]
19. Abdelrahim SS, Hassuna NA, Waly NG, Kotb DN, Abdelhamid H, Zaki S. Coexistence of plasmid-mediated quinolone resistance (PMQR) and extended-spectrum beta-lactamase (ESBL) genes among clinical Pseudomonas aeruginosa isolates in Egypt. BMC Microbiol. 2024;24(1):175. [View at Publisher] [DOI] [PMID] [Google Scholar]
20. Peymani A, Naserpour-Farivar T, Zare E, Azarhoosh KH. Distribution of blaTEM, blaSHV, and blaCTX-M genes among ESBL-producing P. aeruginosa isolated from Qazvin and Tehran hospitals, Iran. J Prev Med Hyg. 2017;58(2):E155. [View at Publisher] [PMID] [Google Scholar]
21. Chen Z, Niu H, Chen G, Li M, Li M, Zhou Y. Prevalence of ESBLs-producing Pseudomonas aeruginosa isolates from different wards in a Chinese teaching hospital. Int J Clin Exp Med. 2015;8(10):19400-05. [View at Publisher] [PMID] [Google Scholar]
22. Bokaeian M, Zahedani SS, Bajgiran MS, Moghaddam AA. Frequency of PER, VEB, SHV, TEM and CTX-M genes in resistant strains of Pseudomonas aeruginosa producing extended spectrum β-lactamases. Jundishapur J Microbiol. 2014;8(1):e13783. [View at Publisher] [DOI] [PMID] [Google Scholar]
23. Fazeli H, Moslehi Z, Irajian G, Salehi M. Determination of Drug resistance patterns and detection of bla-VIM gene in Pseudomonas aeruginosastrains Isolated from burned patients in the Emam Mosa Kazem hospital, Esfahan, Iran (2008-9). Iran J Med Microbiol. 2010;3(4):1-8. [View at Publisher] [Google Scholar]
24. Bahrami M, Mmohammadi-Sichani M, Karbasizadeh V. Prevalence of SHV, TEM, CTX-M and OXA-48 β-Lactamase genes in clinical isolates of Pseudomonas aeruginosa in Bandar-Abbas, Iran. Avicenna J Clin Microbiol Infect. 2018;5(4):86-90. [View at Publisher] [DOI] [Google Scholar]
25. Saleh MAA-jM, Naji HF. Detection of blatem, blactx-m, and blashv genes in clinical isolates of multidrug-resistant pseudomonas aeruginosa. Int J Health Sci . 2022;6(S7):3239-53. [View at Publisher] [DOI] [Google Scholar]
26. Adjei CB, Govinden U, Essack SY, Moodley K. Molecular characterisation of multidrug-resistant Pseudomonas aeruginosa from a private hospital in Durban, South Africa. Southern African Journal of Infectious Diseases. 2018;33(2):38-41. [View at Publisher] [DOI] [Google Scholar]
27. Haghighifar E, Dolatabadi RK, Norouzi F. Prevalence of blaVEB and blaTEM genes, antimicrobial resistance pattern and biofilm formation in clinical isolates of Pseudomonas aeruginosa from burn patients in Isfahan, Iran. Gene Reports. 2021;23:101157. [View at Publisher] [DOI] [Google Scholar]
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