Volume 10, Issue 3 (10-2022)                   Jorjani Biomed J 2022, 10(3): 26-34 | Back to browse issues page

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Mohajer F, Khanzadi S, Hashemi M, Azizzadeh M. Antimicrobial Effect of Chitosan Coating Prepared by Neutral Electrolyzed Water against Inoculated Escherichia coli O157:H7 on Rainbow trout fillets. Jorjani Biomed J 2022; 10 (3) :26-34
URL: http://goums.ac.ir/jorjanijournal/article-1-905-en.html
1- Department of Food Hygiene and Aquaculture, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
2- Department of Food Hygiene and Aquaculture, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran , khanzadi@um.ac.ir
3- Medical Toxicology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran/ Department of Nutrition, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
4- Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
Abstract:   (599 Views)

Background and Objectives: Nowadays, to avoid the use of synthetic preservatives, which do more harm than good, numerous studies are currently focused on using natural ingredients to enhance food product quality and shelf life. Since no study has been conducted on combining coatings with electrolyzed water containing natural antimicrobial compounds, the present study has innovation and priority. In the present study, the effect of chitosan coating prepared through Neutral Electrolyzed Water (NEW) on inhibiting the growth of Escherichia coli O157:H7 inoculated in rainbow trout fillet over 12 days at 4 ˚C was examined.
Material and Methods: Fish samples were allocated into six groups following inoculation with E. coli O157:H7 (final concentration: ~ 105 CFU/g). Treatments included control (CON), distilled water (DW), neutral electrolyzed water (NEW), chitosan 2% (CH), chitosan coating prepared by neutral electrolyzed water (CH/NEW), and neutral electrolyzed water followed by chitosan (NEW+CH). Treatments were kept at low temperature (refrigerator) and counting bacteria was done on 0, 1st, 3rd, 6th, 9th, and 12th days. Data analyses were done through repeated measure ANVOA and Bonferroni post hoc tests.
Results: As indicated by the findings, the reduction rate of EW, CH, CH+WE, and CH/EW samples were 1.04, 1.45, 2.01, and 2.02 log CFU/g compared with the CON, respectively. The highest reduction rate was observed in the CH/NEW sample, due to the antimicrobial activity of chitosan and neutral electrolyzed water.
Conclusion: Chitosan coating can be combined with NEW in fish to increase the safety against pathogenic bacteria and E. coli O157:H7. Therefore, it can suggest the use of these natural antimicrobial compounds in the food industry.

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Type of Article: Original article | Subject: Basic Medical Sciences
Received: 2022/05/29 | Accepted: 2022/07/20 | Published: 2022/08/6

1. Majidiyan N, Hadidi M, Azadikhah D, Moreno A. Protein complex nanoparticles reinforced with industrial hemp essential oil: Characterization and application for shelf-life extension of Rainbow trout fillets. Food Chemistry: X. 2022;13:100202. [DOI] [PMID] [PMCID] [Google Scholar]
2. Fadıloğlu EE, Emir Çoban Ö. Effects of chitosan edible coatings enriched with sumac on the quality and the shelf life of rainbow trout (Oncorhynchus mykiss, Walbaum, 1792) fillets. Journal of Food Safety. 2018;38(6):e12545. [DOI] [Google Scholar]
3. Ozer NP, Demirci A. Inactivation of Escherichia coli O157: H7 and Listeria monocytogenes inoculated on raw salmon fillets by pulsed UV‐light treatment. International journal of food science & technology. 2006;41(4):354-60. [view at publisher] [DOI] [Google Scholar]
4. Bavaro MF. E. coli O157: H7 and other toxigenic strains: the curse of global food distribution. Current gastroenterology reports. 2012;14(4):317-23. [DOI] [PMID] [Google Scholar]
5. Meng J, LeJeune JT, Zhao T, Doyle MP. Enterohemorrhagic Escherichia coli. Food microbiology: Fundamentals and frontiers. 2012:287-309. [DOI] [Google Scholar]
6. Muniesa M, Jofre J, García-Aljaro C, Blanch AR. Occurrence of Escherichia coli O157: H7 and other enterohemorrhagic Escherichia coli in the environment. Environmental science & technology. 2006;40(23):7141-9. [view at publisher] [DOI] [PMID] [Google Scholar]
7. Keykhosravy K, Khanzadi S, Hashemi M, Azizzadeh M. Chitosan-loaded nanoemulsion containing Zataria Multiflora Boiss and Bunium persicum Boiss essential oils as edible coatings: Its impact on microbial quality of turkey meat and fate of inoculated pathogens. International journal of biological macromolecules. 2020;150:904-13. [DOI] [PMID] [Google Scholar]
8. Naga Deepika C, Meghwal M, Prabhakar PK, Singh A, Rani R, Kadeppagari RK. Edible Coatings and Films from Agricultural and Marine Food Wastes. Biotechnology for Zero Waste: Emerging Waste Management Techniques. 2022:543-56. [DOI] [Google Scholar]
9. Shahidi F, Hossain A. Preservation of aquatic food using edible films and coatings containing essential oils: a review. Critical Reviews in Food Science and Nutrition. 2022;62(1):66-105. [view at publisher] [DOI] [PMID] [Google Scholar]
10. Linares MB, Peñaranda I, Iniesta CM, Egea M, Garrido MD. Development of edible gels and films as potential strategy to revalorize entire male pork. Food Hydrocolloids. 2022;123:107182. [DOI] [Google Scholar]
11. Moreira KdS, de Oliveira ALB, de Moura Júnior LS, de Sousa IG, Cavalcante ALG, Neto FS, et al. Taguchi design-assisted co-immobilization of lipase A and B from Candida antarctica onto chitosan: Characterization, kinetic resolution application, and docking studies. Chemical Engineering Research and Design. 2022;177:223-44. [DOI] [Google Scholar]
12. Yüksel Ç, Atalay D, Erge HS. The effects of chitosan coating and vacuum packaging on quality of fresh‐cut pumpkin slices during storage. Journal of Food Processing and Preservation. 2022:e16365. [view at publisher] [DOI] [Google Scholar]
13. Hoa V-B, Song D-H, Seol K-H, Kang S-M, Kim H-W, Kim J-H, et al. Coating with chitosan containing lauric acid (C12: 0) significantly extends the shelf-life of aerobically-Packaged beef steaks during refrigerated storage. Meat Science. 2022;184:108696. [DOI] [PMID] [Google Scholar]
14. Dutta P, Tripathi S, Mehrotra G, Dutta J. Perspectives for chitosan based antimicrobial films in food applications. Food chemistry. 2009;114(4):1173-82. [DOI] [Google Scholar]
15. Sun J, Jiang X, Chen Y, Lin M, Tang J, Lin Q, et al. Recent trends and applications of electrolyzed oxidizing water in fresh foodstuff preservation and safety control. Food Chemistry. 2022;369:130873. [DOI] [PMID] [Google Scholar]
16. Guentzel JL, Lam KL, Callan MA, Emmons SA, Dunham VL. Reduction of bacteria on spinach, lettuce, and surfaces in food service areas using neutral electrolyzed oxidizing water. Food microbiology. 2008;25(1):36-41. [DOI] [PMID] [Google Scholar]
17. Dewi FR, Stanley R, Powell SM, Burke CM. Application of electrolysed oxidising water as a sanitiser to extend the shelf-life of seafood products: a review. Journal of food science and technology. 2017;54(5):1321-32. [DOI] [PMID] [PMCID] [Google Scholar]
18. Posada-Izquierdo GD, Pérez-Rodríguez F, López-Gálvez F, Allende A, Gil MI, Zurera G. Modeling growth of Escherichia coli O157: H7 in fresh-cut lettuce treated with neutral electrolyzed water and under modified atmosphere packaging. International journal of food microbiology. 2014;177:1-8. [DOI] [PMID] [Google Scholar]
19. Pinto L, Ippolito A, Baruzzi F. Control of spoiler Pseudomonas spp. on fresh cut vegetables by neutral electrolyzed water. Food microbiology. 2015;50:102-8. [DOI] [PMID] [Google Scholar]
20. Abadias M, Usall J, Oliveira M, Alegre I, Viñas I. Efficacy of neutral electrolyzed water (NEW) for reducing microbial contamination on minimally-processed vegetables. International journal of food microbiology. 2008;123(1-2):151-8. [DOI] [PMID] [Google Scholar]
21. Ramírez Orejel JC, Cano-Buendía JA. Applications of electrolyzed water as a sanitizer in the food and animal-by products industry. Processes. 2020;8(5):534. [DOI] [Google Scholar]
22. Medina-Gudiño J, Rivera-Garcia A, Santos-Ferro L, Ramirez-Orejel JC, Agredano-Moreno LT, Jimenez-Garcia LF, et al. Analysis of Neutral Electrolyzed Water anti-bacterial activity on contaminated eggshells with Salmonella enterica or Escherichia coli. International journal of food microbiology. 2020;320:108538. [DOI] [PMID] [Google Scholar]
23. Cap M, Rojas D, Fernandez M, Fulco M, Rodriguez A, Soteras T, et al. Effectiveness of short exposure times to electrolyzed water in reducing Salmonella spp and Imidacloprid in lettuce. LWT. 2020;128:109496. [DOI] [Google Scholar]
24. Hernández-Pimentel V, Regalado-González C, Nava-Morales G, Meas-Vong Y, Castañeda-Serrano M, García-Almendárez B. Effect of neutral electrolyzed water as antimicrobial intervention treatment of chicken meat and on trihalomethanes formation. Journal of Applied Poultry Research. 2020;29(3):622-35. [DOI] [Google Scholar]
25. Yaghoubi M, Ayaseh A, Alirezalu K, Nemati Z, Pateiro M, Lorenzo JM. Effect of chitosan coating incorporated with Artemisia fragrans essential oil on fresh chicken meat during refrigerated storage. Polymers. 2021;13(5):716. [DOI] [PMID] [PMCID] [Google Scholar]
26. Afshar Mehrabi F, Sharifi A, Ahvazi M. Effect of chitosan coating containing Nepeta pogonosperma extract on shelf life of chicken fillets during chilled storage. Food Science & Nutrition. 2021;9(8):4517-28. [DOI] [PMID] [PMCID] [Google Scholar]
27. Sayadi M, Langroodi AM, Pourmohammadi K. Combined effects of chitosan coating incorporated with Berberis vulgaris extract and Mentha pulegium essential oil and MAP in the shelf life of turkey meat. Journal of Food Measurement and Characterization. 2021;15(6):5159-69. [DOI] [Google Scholar]
28. Chen X, Chen W, Lu X, Mao Y, Luo X, Liu G, et al. Effect of chitosan coating incorporated with oregano or cinnamon essential oil on the bacterial diversity and shelf life of roast duck in modified atmosphere packaging. Food Research International. 2021:110491. [DOI] [PMID] [Google Scholar]
29. Yang H, Li Q, Xu Z, Ge Y, Zhang D, Li J, et al. Preparation of three-layer flaxseed gum/chitosan/flaxseed gum composite coatings with sustained-release properties and their excellent protective effect on myofibril protein of rainbow trout. International Journal of Biological Macromolecules. 2022;194:510-20. [DOI] [PMID] [Google Scholar]
30. Chaparro-Hernandez S, Ruiz-Cruz S, Marquez-Rios E, Ocano-Higuera VM, Valenzuela-Lopez CC, ORNELAS-PAZ JdJ, et al. Effect of chitosan-carvacrol edible coatings on the quality and shelf life of tilapia (Oreochromis niloticus) fillets stored in ice. Food Science and Technology. 2015;35:734-41. [DOI] [Google Scholar]
31. Luan L, Wu C, Wang L, Li Y, Ishimura G, Yuan C, et al. Protein denaturation and oxidation in chilled hairtail (Trichiutus haumela) as affected by electrolyzed oxidizing water and chitosan treatment. International Journal of Food Properties. 2017;20(sup3):S2696-S707. [DOI] [Google Scholar]
32. Xu G, Tang X, Tang S, You H, Shi H, Gu R. Combined effect of electrolyzed oxidizing water and chitosan on the microbiological, physicochemical, and sensory attributes of American shad (Alosa sapidissima) during refrigerated storage. Food Control. 2014;46:397-402. [DOI] [Google Scholar]
33. Zhou R, Liu Y, Xie J, Wang X. Effects of combined treatment of electrolysed water and chitosan on the quality attributes and myofibril degradation in farmed obscure puffer fish (Takifugu obscurus) during refrigerated storage. Food Chemistry. 2011;129(4):1660-6. [DOI] [Google Scholar]
34. Deza M, Araujo M, Garrido M. Inactivation of Escherichia coli O157: H7, Salmonella enteritidis and Listeria monocytogenes on the surface of tomatoes by neutral electrolyzed water. Letters in applied microbiology. 2003;37(6):482-7. [view at publisher] [DOI] [PMID] [Google Scholar]
35. Soltaninezhad B, Khanzadi S, Hashemi M, Azizzadeh M. The Inhibition of Escherichia coli O157: H7 Inoculated in Hamburger Using a Chitosan/Cellulose Nanofiber Film Containing the Nanoemulsion of Trachyspermum ammi and Bunium persicum Essential Oils. Journal of Human Environment and Health Promotion. 2020;6(1):30-4. [view at publisher] [DOI] [Google Scholar]
36. Arias ML, Monge-Rojas R, Chaves C, Antillón F. Effect of storage temperaturas on growth and survival of Escherichia coli 0157: H7 inoculated in foods from a neotropical environment. Revista de biología tropical. 2001;49(2):517-24. [view at publisher] [Google Scholar]
37. Khanjari A, Akhondzadeh Basti A, Bokaie S, Cheraghi N, Fayazfar S, Ghadami F. Evaluation of the antimicrobial effect of chitosan and whey proteins isolate films containing free and nanoliposomal garlic essential oils against Listeria monocytegenes, E. coli O157: H7 and Staphylococcus aureus. Iranian Journal of Medical Microbiology. 2016;10(5):45-51. [Google Scholar]
38. Ogunniyi AD, Tenzin S, Ferro S, Venter H, Pi H, Amorico T, et al. A pH-neutral electrolyzed oxidizing water significantly reduces microbial contamination of fresh spinach leaves. Food Microbiology. 2021;93:103614. [DOI] [PMID] [Google Scholar]
39. Ogunniyi AD, Dandie CE, Brunetti G, Drigo B, Aleer S, Hall B, et al. Neutral electrolyzed oxidizing water is effective for pre-harvest decontamination of fresh produce. Food Microbiology. 2021;93:103610. [DOI] [PMID] [Google Scholar]
40. Al-Nabulsi A, Osaili T, Sawalha A, Olaimat AN, Albiss BA, Mehyar G, et al. Antimicrobial activity of chitosan coating containing ZnO nanoparticles against E. coli O157: H7 on the surface of white brined cheese. International Journal of Food Microbiology. 2020;334:108838. [DOI] [PMID] [Google Scholar]
41. Shimamura Y, Shinke M, Hiraishi M, Tsuchiya Y, Masuda S. The application of alkaline and acidic electrolyzed water in the sterilization of chicken breasts and beef liver. Food science & nutrition. 2016;4(3):431-40. [DOI] [PMID] [PMCID] [Google Scholar]
42. Kanatt SR, Rao M, Chawla S, Sharma A. Effects of chitosan coating on shelf-life of ready-to-cook meat products during chilled storage. LWT-Food science and technology. 2013;53(1):321-6. [DOI] [Google Scholar]
43. Li J, Zhuang S. Antibacterial activity of chitosan and its derivatives and their interaction mechanism with bacteria: Current state and perspectives. European Polymer Journal. 2020;138:109984. [DOI] [Google Scholar]
44. Shiroodi S, Schwarz MH, Nitin N, Ovissipour R. Efficacy of Nanobubbles Alone or in Combination with Neutral Electrolyzed Water in Removing Escherichia coli O157: H7, Vibrio parahaemolyticus, and Listeria innocua Biofilms. Food and Bioprocess Technology. 2021;14(2):287-97. [DOI] [Google Scholar]
45. Al-Holy MA, Rasco BA. The bactericidal activity of acidic electrolyzed oxidizing water against Escherichia coli O157: H7, Salmonella Typhimurium, and Listeria monocytogenes on raw fish, chicken and beef surfaces. Food Control. 2015;54:317-21. [DOI] [Google Scholar]
46. Rahman S, Wang J, Oh D-H. Synergistic effect of low concentration electrolyzed water and calcium lactate to ensure microbial safety, shelf life and sensory quality of fresh pork. Food control. 2013;30(1):176-83. [DOI] [Google Scholar]

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