Volume 10, Issue 3 (10-2022)                   Jorjani Biomed J 2022, 10(3): 15-25 | Back to browse issues page

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bayani H, Asgharpour H, askari A, Rezaeeshirazi R. The Effect of four Weeks of Continuous Aerobic Training and Starvation on the Expression of Gene Pink1 and Bnibp3 in liver Tissue, liver Enzymes and lipid profile in Wistar Fatty Model Rats. Jorjani Biomed J 2022; 10 (3) :15-25
URL: http://goums.ac.ir/jorjanijournal/article-1-898-en.html
1- Department of Physical Education & Sports sciences, Aliabad katoul Branch, Islamic Azad University, Aliabad katoul, Iran
2- Department of Physical Education & Sports Sciences, Aliabad katoul branch, Islamic Azad University, Aliabad Katoul, Iran , Habibasgharpour@gmail.com
3- Department of Physical Education, Gorgan Branch, Islamic Azad University, Gorgan, Iran
4- Department of Physical Education & sports sciences, Aliabad katoul Branch, Islamic Azad University, Aliabad katoul, Iran
Abstract:   (2303 Views)
Background and Objective: Regular exercise along with calorie intake promotes mitochondrial function by promoting healthy mitochondrial regeneration. The aim of the present study was to investigate the effect of four weeksof continuous aerobic training and starvation on the gene expression of pink1 and bnibp3 (liver mitophagy) in Wistar fat rats.
Materials and Methods: The present study used an experimental design. Thirty 18-week-old fatty, maleWistar rats with an average body weight of 348±25.53 purchased from the Pasteur Institute of Iran were selected as the research sample. After one week of familiarity with the laboratory environment, these fatty animals were randomly divided into 6 groups of 5, control (n=5) and experimental (n=25), including control, starvation, starvation and 3 days of exercise, starvation and 5 days of exercise, 3 days of exercise, 5 days of exercise groups.
Results: According to the statistical results of one-way analysis of variance, there was a significant decrease in triglyceride, cholesterol, liver enzymes ALT, AST in all groups compared to the control group. Furthermore, there was a significant increase in pink1 and bnibp3 gene expression in starvation group and starvation groups of 3 and 5 days of training compared to the control group.
Conclusion: Four weeksof continuous aerobic training and starvation combined and alone were able to significantly reduce the status of blood lipids and liver enzymes in fatty model rats. Also, the starvation group and starvation groups along with exercise increased the activity of removing damaged mitochondria by increasing the activity of pink1 and bnibp3 genes compared to the control group.
 
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Type of Article: Original article | Subject: Health
Received: 2022/04/20 | Accepted: 2022/07/19 | Published: 2022/08/6

References
1. Aki T, Uemura K. Cell Death and Survival Pathways Involving ATM Protein Kinase. Genes. 2021 Oct;12(10):1581. [DOI] [PMID] [PMCID] [Google Scholar]
2. Alex S, Boss A, Heerschap A, Kersten S. Exercise training improves liver steatosis in mice. Nutrition & metabolism. 2015 Dec;12(1):1-1. [DOI] [PMID] [PMCID] [Google Scholar]
3. Baldini F, Portincasa P, Grasselli E, Damonte G, Salis A, Bonomo M, Florio M, Serale N, Voci A, Gena P, Vergani L. Aquaporin-9 is involved in the lipid-lowering activity of the nutraceutical silybin on hepatocytes through modulation of autophagy and lipid droplets composition. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids. 2020 Mar 1;1865(3):158586. [DOI] [PMID] [Google Scholar]
4. Benedict M, Zhang X. Non-alcoholic fatty liver disease: An expanded review. World journal of hepatology. 2017 Jun 8;9(16):715. [DOI] [PMID] [PMCID] [Google Scholar]
5. Campbell JE, Newgard CB. Mechanisms controlling pancreatic islet cell function in insulin secretion. Nature reviews Molecular cell biology. 2021 Feb;22(2):142-58. [DOI] [PMID] [PMCID] [Google Scholar]
6. Chalasani N, Younossi Z, Lavine JE, Charlton M, Cusi K, Rinella M, Harrison SA, Brunt EM, Sanyal AJ. The diagnosis and management of nonalcoholic fatty liver disease: practice guidance from the American Association for the Study of Liver Diseases. Hepatology. 2018 Jan;67(1):328-57. [DOI] [PMID] [Google Scholar]
7. Chen YY, Yeh MM. Non-alcoholic fatty liver disease: A review with clinical and pathological correlation. Journal of the Formosan Medical Association. 2021 Jan 1;120(1):68-77. [DOI] [PMID] [Google Scholar]
8. Cho EJ, Yu SJ, Jung GC, Kwak MS, Yang JI, Yim JY, Chung GE. Body weight gain rather than body weight variability is associated with increased risk of nonalcoholic fatty liver disease. Scientific reports. 2021 Jul 13;11(1):1-7. [view at publisher] [DOI] [PMID] [PMCID] [Google Scholar]
9. Chun SK, Lee S, Yang MJ, Leeuwenburgh C, Kim JS. Exercise-induced autophagy in fatty liver disease. Exercise and sport sciences reviews. 2017 Jul;45(3):181. [DOI] [PMID] [PMCID] [Google Scholar]
10. Doblado L, Lueck C, Rey C, Samhan-Arias AK, Prieto I, Stacchiotti A, Monsalve M. Mitophagy in human diseases. International journal of molecular sciences. 2021 Jan;22(8):3903. [DOI] [PMID] [PMCID] [Google Scholar]
11. Drake JC, Laker RC, Wilson RJ, Zhang M, Yan Z. Exercise-induced mitophagy in skeletal muscle occurs in the absence of stabilization of Pink1 on mitochondria. Cell Cycle. 2019 Jan 2;18(1):1-6. [DOI] [PMID] [PMCID] [Google Scholar]
12. Friedman SL, Neuschwander-Tetri BA, Rinella M, Sanyal AJ. Mechanisms of NAFLD development and therapeutic strategies. Nature medicine. 2018 Jul;24(7):908-22. [view at publisher] [DOI] [PMID] [PMCID] [Google Scholar]
13. Furkan M, Alam MT, Rizvi A, Khan K, Ali A, Naeem A. Aloe emodin, an anthroquinone from Aloe vera acts as an anti-aggregatory agent to the thermally aggregated hemoglobin. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 2017 May 15; 179:188-93. [DOI] [PMID] [Google Scholar]
14. Gao Y, Lu J, Liu X, Liu J, Ma Q, Shi Y, Su H. Effect of Long-Term Exercise on Liver Lipid Metabolism in Chinese Patients With NAFLD: A Systematic Review and Meta-Analysis. Frontiers in physiology. 2021:1892. [DOI] [PMID] [PMCID] [Google Scholar]
15. Ge P, Dawson VL, Dawson TM. PINK1 and Parkin mitochondrial quality control: A source of regional vulnerability in Parkinson's disease. Molecular neurodegeneration. 2020 Dec;15(1):1-8. [DOI] [PMID] [PMCID] [Google Scholar]
16. Ge Y, Zhou M, Chen C, Wu X, Wang X. Role of AMPK mediated pathways in autophagy and aging. Biochimie. 2022 Apr 1;195:100-13. [DOI] [PMID] [Google Scholar]
17. Godoy-Matos AF, Silva Júnior WS, Valerio CM. NAFLD as a continuum: from obesity to metabolic syndrome and diabetes. Diabetology & Metabolic Syndrome. 2020 Dec;12(1):1-20. [DOI] [PMID] [PMCID] [Google Scholar]
18. Hundertmark J, Tacke F. How effective are nonalcoholic fatty liver disease models for drug discovery? Expert Opinion on Drug Discovery. 2020 Nov 1;15(11):1237-40. [DOI] [PMID] [Google Scholar]
19. Im YR, Hunter H, de Gracia Hahn D, Duret A, Cheah Q, Dong J, Fairey M, Hjalmarsson C, Li A, Lim HK, McKeown L. A Systematic Review of Animal Models of NAFLD Finds High‐Fat, High‐Fructose Diets Most Closely Resemble Human NAFLD. Hepatology. 2021 Oct;74(4):1884-901. [DOI] [PMID] [Google Scholar]
20. Jiménez-Cortegana C, García-Galey A, Tami M, Del Pino P, Carmona I, López S, Alba G, Sánchez-Margalet V. Role of leptin in non-alcoholic fatty liver disease. Biomedicines. 2021 Jul;9(7):762. [DOI] [PMID] [PMCID] [Google Scholar]
21. Kim J, Kundu M, Viollet B, Guan KL. AMPK and mTOR regulate autophagy through direct phosphorylation of Ulk1. Nature cell biology. 2011 Feb;13(2):132-41. [view at publisher] [DOI] [PMID] [PMCID] [Google Scholar]
22. Kleiner DE, Brunt EM, Wilson LA, Behling C, Guy C, Contos M, Cummings O, Yeh M, Gill R, Chalasani N, Neuschwander-Tetri BA. Association of histologic disease activity with progression of nonalcoholic fatty liver disease. JAMA network open. 2019 Oct 2;2(10): e1912565-. [DOI] [PMID] [PMCID] [Google Scholar]
23. Koch LK, Yeh MM. Nonalcoholic fatty liver disease (NAFLD): diagnosis, pitfalls, and staging. Annals of diagnostic pathology. 2018 Dec 1; 37:83-90. [DOI] [PMID] [Google Scholar]
24. Lampert MA, Orogo AM, Najor RH, Hammerling BC, Leon LJ, Wang BJ, Kim T, Sussman MA, Gustafsson ÅB. BNIP3L/NIX and FUNDC1-mediated mitophagy is required for mitochondrial network remodeling during cardiac progenitor cell differentiation. Autophagy. 2019 Jul 3;15(7):1182-98. [DOI] [PMID] [PMCID] [Google Scholar]
25. López-Sánchez GN, Dóminguez-Pérez M, Uribe M, Chávez-Tapia NC, Nuño-Lámbarri N. Non-alcoholic fatty liver disease and microRNAs expression, how it affects the development and progression of the disease. Annals of hepatology. 2021 Mar 1;21:100212. [DOI] [PMID] [Google Scholar]
26. Ma X, McKeen T, Zhang J, Ding WX. Role and mechanisms of mitophagy in liver diseases. Cells. 2020 Apr;9(4):837. [DOI] [PMID] [PMCID] [Google Scholar]
27. Martínez-Uña M, López-Mancheño Y, Diéguez C, Fernández-Rojo MA, Novelle MG. Unraveling the role of leptin in liver function and its relationship with liver diseases. International journal of molecular sciences. 2020 Jan;21(24):9368. [DOI] [PMID] [PMCID] [Google Scholar]
28. Muscella A, Stefano E, Marsigliante S. The effects of exercise training on lipid metabolism and coronary heart disease. American Journal of Physiology-Heart and Circulatory Physiology. 2020 Jul 1;319(1):H76-88. [DOI] [PMID] [Google Scholar]
29. Paik JM, Golabi P, Younossi Y, Srishord M, Mishra A, Younossi ZM. The growing burden of disability related to nonalcoholic fatty liver disease: data from the global burden of disease 2007‐2017. Hepatology communications. 2020 Dec;4(12):1769-80. [DOI] [PMID] [PMCID] [Google Scholar]
30. Perdomo CM, Frühbeck G, Escalada J. Impact of nutritional changes on nonalcoholic fatty liver disease. Nutrients. 2019 Mar;11(3):677. [DOI] [PMID] [PMCID] [Google Scholar]
31. Shen W, Zhang X, Fu X, Fan J, Luan J, Cao Z, Yang P, Xu Z, Ju D. A novel and promising therapeutic approach for NSCLC: recombinant human arginase alone or combined with autophagy inhibitor. Cell death & disease. 2017 Mar;8(3): e2720-. [DOI] [PMID] [PMCID] [Google Scholar]
32. Shirakabe A, Zhai P, Ikeda Y, Saito T, Maejima Y, Hsu CP, Nomura M, Egashira K, Levine B, Sadoshima J. Drp1-dependent mitochondrial autophagy plays a protective role against pressure overload-induced mitochondrial dysfunction and heart failure. Circulation. 2016 Mar 29;133(13):1249-63. [DOI] [PMID] [PMCID] [Google Scholar]
33. Singh A, Azad M, Shymko MD, Henson ES, Katyal S, Eisenstat DD, Gibson SB. The BH3 only Bcl-2 family member BNIP3 regulates cellular proliferation. PloS one. 2018 Oct 11;13(10): e0204792. [view at publisher] [DOI] [PMID] [PMCID] [Google Scholar]
34. Stevanović J, Beleza J, Coxito P, Ascensão A, Magalhães J. Physical exercise and liver "fitness": Role of mitochondrial function and epigenetics-related mechanisms in non-alcoholic fatty liver disease. Molecular metabolism. 2020 Feb 1;32:1-4. [DOI] [PMID] [PMCID] [Google Scholar]
35. Velázquez KT, Enos RT, Bader JE, Sougiannis AT, Carson MS, Chatzistamou I, Carson JA, Nagarkatti PS, Nagarkatti M, Murphy EA. Prolonged high-fat-diet feeding promotes non-alcoholic fatty liver disease and alters gut microbiota in mice. World journal of hepatology. 2019 Aug 27;11(8):619. [DOI] [PMID] [PMCID] [Google Scholar]
36. Wang H, Jiang T, Li W, Gao NA, Zhang T. Resveratrol attenuates oxidative damage through activating mitophagy in an in vitro model of Alzheimer's disease. Toxicology letters. 2018 Jan 5; 282:100-8. [DOI] [PMID] [Google Scholar]
37. Xie Y, Liu J, Kang R, Tang D. Mitophagy receptors in tumor biology. Frontiers in Cell and Developmental Biology. 2020:1315. [DOI] [PMID] [PMCID] [Google Scholar]
38. Younossi Z, Anstee QM, Marietti M, Hardy T, Henry L, Eslam M, George J, Bugianesi E. Global burden of NAFLD and NASH: trends, predictions, risk factors and prevention. Nature reviews Gastroenterology & hepatology. 2018 Jan;15(1):11-20. [view at publisher] [DOI] [PMID] [Google Scholar]
39. Younossi Z, Tacke F, Arrese M, Chander Sharma B, Mostafa I, Bugianesi E, Wai‐Sun Wong V, Yilmaz Y, George J, Fan J, Vos MB. Global perspectives on nonalcoholic fatty liver disease and nonalcoholic steatohepatitis. Hepatology. 2019 Jun;69(6):2672-82. [DOI] [PMID] [Google Scholar]
40. Younossi ZM, Marchesini G, Pinto-Cortez H, Petta S. Epidemiology of nonalcoholic fatty liver disease and nonalcoholic steatohepatitis: implications for liver transplantation. Transplantation. 2019 Jan 1;103(1):22-7. [DOI] [PMID] [Google Scholar] [Google Scholar]
41. Younossi ZM. Non-alcoholic fatty liver disease-a global public health perspective. Journal of hepatology. 2019 Mar 1;70(3):531-44. [DOI] [PMID] [Google Scholar]
42. Yu X, Meng Z, Fang T, Liu X, Cheng Y, Xu L, Liu X, Li X, Xue M, Li T, Sun B. Empagliflozin Inhibits Hepatic Gluconeogenesis and Increases Glycogen Synthesis by AMPK/CREB/GSK3β Signalling Pathway. Frontiers in Physiology. 2022:328. [DOI] [PMID] [PMCID] [Google Scholar]
43. Zhang W, Ma Q, Siraj S, Ney PA, Liu J, Liao X, Yuan Y, Li W, Liu L, Chen Q. Nix-mediated mitophagy regulates platelet activation and life span. Blood advances. 2019 Aug 13;3(15):2342-54. [DOI] [PMID] [PMCID] [Google Scholar]
44. Zhang X, Liang T, Yang W, Zhang L, Wu S, Yan C, Li Q. Astragalus membranaceus injection suppresses production of interleukin-6 by activating autophagy through the AMPK-mTOR pathway in lipopolysaccharide-stimulated macrophages. Oxidative medicine and cellular longevity. 2020 Jul 4;2020. [view at publisher] [DOI] [PMID] [PMCID]
45. Zhou JH, Cai JJ, She ZG, Li HL. Noninvasive evaluation of nonalcoholic fatty liver disease: Current evidence and practice. World journal of gastroenterology. 2019 Mar 21;25(11):1307. [DOI] [PMID] [PMCID] [Google Scholar]

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