Volume 10, Issue 2 (5-2022)                   Jorjani Biomed J 2022, 10(2): 24-35 | Back to browse issues page

XML Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Omomi M, Taghian F, Sharifi G. Endurance Training and Consumption of Hydroalcoholic Zingiber Officinale Extract Regulated PPARγ, PGC1-ɑ/TNF-ɑ Expression Level in Myocardial Infarction Rats. Jorjani Biomed J. 2022; 10 (2) :24-35
URL: http://goums.ac.ir/jorjanijournal/article-1-891-en.html
1- Department of Sports Physiology, Faculty of Sports Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran.
2- Department of Sports Physiology, Faculty of Sports Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran. , f_taghian@yahoo.com
Abstract:   (826 Views)
Background and objectives: Zingiber officinale extract can control cardiovascular risk factors. Moreover, endurance training may effectively rehabilitate myocardial infarction by strengthening the myocardial muscle tissue. In-silico analysis identified essential genes involved in the heart damage process based on data from the DisGeNET database. Hence, we estimated the affinity of chemical and bioactive molecules for PPARγ. Therefore, this study aimed to investigate the effect of endurance exercise alone or combined with Zingiber officinale extract on Myocardial infarction rats.
Material and Methods: Twenty-five male rats were randomly divided into five groups, including (1) group of myocardial infarctions (MI) induced by subcutaneous injection of isoproterenol, (2) myocardial infarction+exercise (MI+EX), (3) myocardial infarction+Zingiber Officinale extraction administered orally (MI+GE), (4) myocardial infarction+exercise+Zingiber Officinale extract (MI+EX+GE), and (5) Control group. The qPCR-Real Time technique was used to measure the expression of PGC1-ɑ, PPARγ, and TNF-ɑ genes. We evaluated the concentration of Troponin-1 as a vital myocardial ischemia marker.
Results: In bioinformatics analysis, we found that the PPARγ, PGC1-ɑ, and TNF-ɑ pathways were critical in heart injury. Also, the effects of Zingiber officinale on heart tissue were detected through PPARγ by drug design. Endurance training combined with Zingiber officinale consumption reduced the expression of TNF-ɑ, Troponin-1 and increased the PGC1-ɑ, PPARγ genes. Furthermore, consumption of Zingiber officinale extraction improved the levels of PGC1-ɑ, PPARγ, TNF-ɑ, and Troponin-1.
Conclusion: Our data indicated that six weeks of endurance training and consumption of Zingiber officinale extract could reduce the relative expression of the TNF-ɑ and significantly increase the level of PGC1-ɑ, PPARγ.

Full-Text [PDF 870 kb]   (256 Downloads) |   |   Full-Text (HTML)  (86 Views)  
 
 Endurance training can improve the PGC1-ɑ, PPAR-γ, and TNF-ɑ expression in MI.
 In MI status, Zingiber officinale extract can regulate PGC1-ɑ, PPAR-γ, and TNF-ɑ expression.
 Endurance training and Zingiber officinale extract can ameliorate the function of mitochondria.

 

Type of Article: Original article | Subject: Molecular Sciences
Received: 2022/02/21 | Accepted: 2022/05/21 | Published: 2022/05/31

References
1. Jabbar A, Ingoe L, Thomas H, Carey P, Junejo S, Addison C, et al. Prevalence, predictors and outcomes of thyroid dysfunction in patients with acute myocardial infarction: the ThyrAMI-1 study. Journal of Endocrinological Investigation. 2021;44(6):1209-18. [DOI] [PMID] [PMCID]
2. Vähätalo JH, Huikuri HV, Holmström LT, Kenttä TV, Haukilahti MAE, Pakanen L, et al. Association of silent myocardial infarction and sudden cardiac death. JAMA cardiology. 2019;4(8):796-802. [DOI] [PMID] [PMCID]
3. Amran AZ, Jantan I, Dianita R, Buang F. Protective effects of the standardized extract of Zingiber officinale on myocardium against isoproterenol-induced biochemical and histopathological alterations in rats. Pharmaceutical biology. 2015;53(12):1795-802. [DOI] [PMID]
4. Zarei S, Taghian F, Sharifi G, Abedi H. Novel prevention insights into depletion of oxidative stress status through regular exercise and grape seed effective substance in heart ischemia rat model. Food Science & Nutrition. 2022. [DOI] [PMID] [PMCID]
5. White HD, Chew DP. Acute myocardial infarction. The Lancet. 2008;372(9638):570-84. [DOI]
6. Hajibabaie F, Kouhpayeh S, Mirian M, Rahimmanesh I, Boshtam M, Sadeghian L, et al. MicroRNAs as the actors in the atherosclerosis scenario. Journal of physiology and biochemistry. 2020;76(1):1-12. [DOI] [PMID]
7. Yao X, Yue X, Yang Y, Zhang G. Effect of Low Molecular Weight Heparin Calcium Combined with Argatroban on the Treatment, Vascular Endothelial Function, Inflammatory Factors and Serum Neurological Function in Patients with Acute Cerebral Infarction. 2022. [DOI]
8. Daniyal M, Wang W. Molecular pharmacology of inflammation: Medicinal plants as antiinflammatory agents. Inflammation and Natural Products: Elsevier; 2021. p. 21-63. [DOI]
9. Tasneem S, Liu B, Li B, Choudhary MI, Wang W. Molecular pharmacology of inflammation: Medicinal plants as anti-inflammatory agents. Pharmacological research. 2019;139:126-40. [DOI] [PMID]
10. Shahrajabian MH, Sun W, Cheng Q. The power of natural Chinese medicine, ginger and ginseng root in an organic life. Middle-East Journal of Scientific Research. 2019;27(1):64-71.
11. Han YA, Song CW, Koh WS, Yon GH, Kim YS, Ryu SY, et al. Anti‐inflammatory effects of the Zingiber officinale roscoe constituent 12‐dehydrogingerdione in lipopolysaccharide‐stimulated Raw 264.7 cells. Phytotherapy Research. 2013;27(8):1200-5. [DOI] [PMID]
12. Mao Q-Q, Xu X-Y, Cao S-Y, Gan R-Y, Corke H, Li H-B. Bioactive compounds and bioactivities of ginger (Zingiber officinale Roscoe). Foods. 2019;8(6):185. [DOI] [PMID] [PMCID]
13. Stoner GD. Ginger: is it ready for prime time? Cancer Prevention Research. 2013;6(4):257-62. [DOI] [PMID]
14. Xie F, Wu Y-Y, Duan G-J, Wang B, Gao F, Wei P-F, et al. Anti-Myocardial Ischemia Reperfusion Injury Mechanism of Dried Ginger-Aconite Decoction Based on Network Pharmacology. Frontiers in pharmacology. 2021;12. [DOI] [PMID] [PMCID]
15. Ji K, Fang L, Zhao H, Li Q, Shi Y, Xu C, et al. Ginger oleoresin alleviated γ-ray irradiation-induced reactive oxygen species via the Nrf2 protective response in human mesenchymal stem cells. Oxidative Medicine and Cellular Longevity. 2017;2017. [DOI] [PMID] [PMCID]
16. Akinyemi AJ, Ademiluyi AO, Oboh G. Aqueous extracts of two varieties of ginger (Zingiber officinale) inhibit Angiotensin i-converting enzyme, iron (II), and sodium nitroprusside-induced lipid peroxidation in the rat heart in vitro. Journal of medicinal food. 2013;16(7):641-6. [DOI] [PMID]
17. Suk S, Kwon GT, Lee E, Jang WJ, Yang H, Kim JH, et al. Gingerenone A, a polyphenol present in ginger, suppresses obesity and adipose tissue inflammation in high‐fat diet‐fed mice. Molecular nutrition & food research. 2017;61(10):1700139. [DOI] [PMID] [PMCID]
18. Verma VK, Malik S, Narayanan SP, Mutneja E, Sahu AK, Bhatia J, et al. Role of MAPK/NF-κB pathway in cardioprotective effect of Morin in isoproterenol induced myocardial injury in rats. Molecular biology reports. 2019;46(1):1139-48. [DOI] [PMID]
19. Abedpoor N, Taghian F, Ghaedi K, Niktab I, Safaeinejad Z, Rabiee F, et al. PPARγ/Pgc-1α-Fndc5 pathway up-regulation in gastrocnemius and heart muscle of exercised, branched chain amino acid diet fed mice. Nutrition & metabolism. 2018;15(1):1-15. [DOI] [PMID] [PMCID]
20. Wagner K-D, Wagner N. PPARs and myocardial infarction. International Journal of Molecular Sciences. 2020;21(24):9436. [DOI] [PMID] [PMCID]
21. Abedpoor N, Taghian F, Hajibabaie F. Physical activity ameliorates the function of organs via adipose tissue in metabolic diseases. Acta histochemica. 2022;124(2):151844. [DOI] [PMID]
22. Kleinbongard P, Heusch G, Schulz R. TNFα in atherosclerosis, myocardial ischemia/reperfusion and heart failure. Pharmacology & therapeutics. 2010;127(3):295-314. [DOI] [PMID]
23. Rodríguez A, Becerril S, Hernández-Pardos AW, Frühbeck G. Adipose tissue depot differences in adipokines and effects on skeletal and cardiac muscle. Current Opinion in Pharmacology. 2020;52:1-8. [DOI] [PMID]
24. Pakravan G, Peymani M, Abedpoor N, Safaeinejad Z, Yadegari M, Derakhshan M, et al. Antiapoptotic and anti‐inflammatory effects of Pparγ agonist, pioglitazone, reversed Dox‐induced cardiotoxicity through mediating of miR‐130a downregulation in C57BL/6 mice. Journal of Biochemical and Molecular Toxicology. 2022:e23041. [DOI] [PMID]
25. Xu X, Wan W, Ji L, Lao S, Powers AS, Zhao W, et al. Exercise training combined with angiotensin II receptor blockade limits post-infarct ventricular remodelling in rats. Cardiovascular research. 2008;78(3):523-32. [DOI] [PMID]
26. Xu X, Zhao W, Lao S, Wilson BS, Erikson JM, Zhang JQ. Effects of exercise and L-arginine on ventricular remodeling and oxidative stress. Medicine and science in sports and exercise. 2010;42(2):346. [DOI] [PMID] [PMCID]
27. Wang X, Zhang M, Woloshun RR, Yu Y, Lee JK, Flores SR, et al. Oral administration of ginger-derived lipid nanoparticles and Dmt1 siRNA potentiates the effect of dietary iron restriction and mitigates pre-existing iron overload in hamp KO mice. Nutrients. 2021;13(5):1686. [DOI] [PMID] [PMCID]
28. Abdi T, Mahmoudabady M, Marzouni HZ, Niazmand S, Khazaei M. Ginger (Zingiber Officinale Roscoe) extract protects the heart against inflammation and fibrosis in diabetic rats. Canadian Journal of Diabetes. 2021;45(3):220-7. [DOI] [PMID]
29. Sharma M, Kishore K, Gupta SK, Joshi S, Arya DS. Cardioprotective potential of Ocimum sanctum in isoproterenol induced myocardial infarction in rats. Molecular and cellular biochemistry. 2001;225(1):75-83. [DOI] [PMID]
30. Piñero J, Saüch J, Sanz F, Furlong LI. The DisGeNET cytoscape app: Exploring and visualizing disease genomics data. Computational and structural biotechnology journal. 2021;19:2960-7. [DOI] [PMID] [PMCID]
31. Szklarczyk D, Gable AL, Nastou KC, Lyon D, Kirsch R, Pyysalo S, et al. The STRING database in 2021: customizable protein-protein networks, and functional characterization of user-uploaded gene/measurement sets. Nucleic acids research. 2021;49(D1):D605-D12. [DOI] [PMID] [PMCID]
32. Xie Z, Bailey A, Kuleshov MV, Clarke DJ, Evangelista JE, Jenkins SL, et al. Gene set knowledge discovery with enrichr. Current protocols. 2021;1(3):e90. [DOI] [PMID] [PMCID]
33. Burley SK, Bhikadiya C, Bi C, Bittrich S, Chen L, Crichlow GV, et al. RCSB Protein Data Bank: powerful new tools for exploring 3D structures of biological macromolecules for basic and applied research and education in fundamental biology, biomedicine, biotechnology, bioengineering and energy sciences. Nucleic acids research. 2021;49(D1):D437-D51. [DOI] [PMID] [PMCID]
34. Kim S, Chen J, Cheng T, Gindulyte A, He J, He S, et al. PubChem in 2021: new data content and improved web interfaces. Nucleic acids research. 2021;49(D1):D1388-D95. [DOI] [PMID] [PMCID]
35. Dallakyan S, Olson AJ. Small-molecule library screening by docking with PyRx. Chemical biology: Springer; 2015. p. 243-50. [DOI] [PMID]
36. Tian M, Yuan Y-C, Li J-Y, Gionfriddo MR, Huang R-C. Tumor necrosis factor-α and its role as a mediator in myocardial infarction: a brief review. Chronic Diseases and Translational Medicine. 2015;1(1):18-26. [DOI] [PMID] [PMCID]
37. de Las Heras N, Valero-Muñoz M, Martín-Fernández B, Ballesteros S, López-Farré A, Ruiz-Roso B, et al. Molecular factors involved in the hypolipidemic-and insulin-sensitizing effects of a ginger (Zingiber officinale Roscoe) extract in rats fed a high-fat diet. Applied Physiology, Nutrition, and Metabolism. 2017;42(2):209-15. [DOI] [PMID]

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2022 CC BY-NC 4.0 | Jorjani Biomedicine Journal

Designed & Developed by : Yektaweb