Volume 9, Issue 3 (9-2021)                   Jorjani Biomed J 2021, 9(3): 42-48 | Back to browse issues page

XML Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Hashempour E, Fozouni L, Ahani Azari A. Evaluating Susceptibility of Environmental Isolates of Pseudomonas aeruginosa to Chlorhexidine Gluconate and Aqueous Extract of Peganum harmala. Jorjani Biomed J. 2021; 9 (3) :42-48
URL: http://goums.ac.ir/jorjanijournal/article-1-837-en.html
1- Department of Biology, Gorgan Branch, Islamic Azad University, Gorgan, Iran
2- Department of Biology, Gorgan Branch, Islamic Azad University, Gorgan, Iran , L.fozouni@gorganiau.ac.ir
Abstract:   (527 Views)
BBackground and Objective: Pseudomonas aeruginosa is an important case of nosocomial infections and a major health problem. The increased emergence of resistance to antibiotics and disinfectants among these bacteria, necessitates the production of new antimicrobials with wider activity and low toxicity. This study was carried out to evaluate inhibitory effects of chlorhexidine gluconate and extract of Peganum harmala on multi-drug resistant (MDR) P. aeruginosa.
Material and Methods: 39 P. aeruginosa strains were isolated from 114 environmental samples. After identifying MDR strains, minimal inhibitory concentration (MIC) of chlorhexidine gluconate (20%) and P. harmala extract against the isolates was determined by broth microdilution method. 
Results: Overall, 27 (69.2%) P. aeruginosa isolates resistant to quinolones, aminoglycoside, cephems and carbapenems were reported as MDR strains. The MIC of chlorhexidine gluconate was ≥ 1000μg/ml, which was 4-fold higher than the MIC of P. harmala extract (MIC=500μg/ml). MIC of chlorhexidine gluconate and P. harmala extract against P. aeruginosa isolates differed significantly (P=0.01).
Conclusion: Compared to chlorhexidine gluconate, P. harmala extract has a higher antibacterial effect on MDR P. aeruginosa isolates from environment. Further research is required to verify the efficacy of this plant extract for disinfection of equipment in clinics and local kitchens.
Full-Text [PDF 468 kb]   (92 Downloads) |   |   Full-Text (HTML)  (49 Views)  
Type of Article: Original article | Subject: Health
Received: 2021/06/11 | Accepted: 2021/08/31 | Published: 2021/09/29

References
1. Furtado GH, Gales AC, Perdiz LB, Santos AF, Wey SB, Medeiros EA. Risk factors for hospital-acquired pneumonia caused by imipenem-resistant Pseudomonas aeruginosa in an intensive care unit. Anaesth Intensive Care. 2010; 38(6):994-1001. [view at publisher] [DOI] [PMID] [Google Scholar]
2. Walker TS. Microbiology. philadelphia.W.B.Saunders Company.2003.
3. Pachori P, Gothalwal R, Gandhi P. Emergence of antibiotic resistance Pseudomonas aeruginosa in intensive care unit; a critical review. Genes & Diseases. 2019; 6: 109-119. [view at publisher] [DOI] [PMID] [PMCID] [Google Scholar]
4. Yayan J, Ghebremedhin B, Rasche K. Antibiotic Resistance of Pseudomonas aeruginosa in Pneumonia at a Single University Hospital Center in Germany over a 10-Year Period. PLOS ONE. 2015:1-20. [DOI] [PMID] [PMCID]
5. De Francesco MA, Ravizzola G, Peroni L, Bonfanti C, Manca N. Prevalence of multidrug-resistant Acinetobacter baumannii and Pseudomonas aeruginosa in an Italian hospital. J Infect Public Health. 2013; 6 (3):179-185. [view at publisher] [DOI] [PMID] [Google Scholar]
6. E Wand M, Bock LJ, Bonney L C, Sutton JM.6. Mechanisms of Increased Resistance to Chlorhexidine and Cross-Resistance to Colistin following Exposure of Klebsiella pneumoniae Clinical Isolates to Chlorhexidine. Antimicrob Agents Chemother . 2016; 61(1): e 01162-16. [view at publisher] [DOI] [PMID] [PMCID] [Google Scholar]
7. Moradi M.T, Karimi A, Rafieian-Kopaei M, Fotouhi F. In vitro antiviral effects of Peganum harmala seed extract and its total alkaloids against Influenza virus. Microb Pathog. 2017; 110: 42-49. [view at publisher] [DOI] [PMID] [Google Scholar]
8. Berrougui H, Martin-Cordero C, Khalil A, Hmamouchi M, Ettaib A, Marhuenda E ,Herrera MD. Vasorelaxant effects of harmine and harmaline extracted from Peganum harmala seeds in isolated rat aorta. Pharmacol Res.2006; 54:150-157. [view at publisher] [DOI] [PMID] [Google Scholar]
9. Astulla A, Zaima K, Matsuno Y, Hirasawa Y, Ekasari W, Widyawaruyanti A, Zaini NC ,Morita H. Alkaloids from the seeds of Peganum harmala showing antiplasmodial and vasorelaxant activities. J Nat Med.2008; 62:470-472. [view at publisher] [DOI] [PMID] [Google Scholar]
10. Amiri R, Fozouni L. Antibacterial effects of extracts from Peganum harmala seeds on drug-resistant clinical isolates of Acinetobacter baumannii in the north of Iran. Jundishapur J. Nat. Pharm. Prod.2019; 15(2): e92426. [DOI]
11. Weinstein MP, Campeau S, Sharon CK, Bobenchik AM, Galas MF, Gold H, et al. Performance standards for antimicrobial susceptibility testing m100. Wayne (PA): Clinical and Laboratory Standards Institute; 2020. p. 83-5.
12. Abasgholizadeh M, Fozouni L. Antagonistic Effect of Probiotics on Drug Resistant Pseudomonas aeruginosa Isolated from Burn Wound Infection. Int. J. Hosp. Res. 2017;6 (2):104-110. [view at publisher] [Google Scholar]
13. Tacconelli E, Tumbarello M, Bertagnolio S, Citton R, Spanu T, Fadda G, et al. Multidrug-resistant Pseudomonas aeruginosa bloodstream infections: analysis of trends in prevalence and epidemiology. Emerg Infect Dis. 2002; 8(2): 220-221. [view at publisher] [DOI] [PMID] [PMCID] [Google Scholar]
14. Saderi H, Owlia P. Detection of Multidrug Resistant (MDR) and Extremely Drug Resistant (XDR) Pseudomonas aeruginosa Isolated from Patients in Tehran, Iran. Iran J Pathol. 2015; 10(4): 265 - 271. [PMID] [Google Scholar]
15. Gomes BP, Ferraz CC, Vianna ME, Berber VB, Teixeira FB, Souza-Filho FJ. In vitro antimicrobial activity of several concentrations of sodium hypochlorite and chlorhexidine gluconate in the elimination of enterococcus faecalis. Int Endod J. 2001;34:424-428. [view at publisher] [DOI] [PMID] [Google Scholar]
16. Darvishi Khezri H, Haidari Gorji MA, Morad A, Gorji H. Comparison of the antibacterial effects of matrica & Persica™ and chlorhexidine gluconate mouthwashes in mechanically ventilated ICU patients: a double blind randomized clinical trial .Rev Chilena Infectol. 2013;30(4):361-373. [DOI] [PMID] [Google Scholar]
17. Mehmandoust P, Farhadmollashahi N, Ghasemi A. Antibacterial efficacy of lavandula officinalis extract, sodium hypochlorite and chlorhexidine gluconate solutions as root canal irrigations: A comparative analysis . Caspian J Dent Res. 2016; 5 (1):14-20. URL: http://cjdr.ir/article-1-175-en.html. [Google Scholar]
18. Bonez PC, Dos Santos Alves CF, Dalmolin TV, Agertt VA, Mizdal CR, Flores Vda C RC .Chlorhexidine activity against bacterial biofilms. Am J Infect Control. 2013;41(12):e119-122. [view at publisher] [DOI] [PMID] [Google Scholar]
19. Baradari AG, Khezri HD, Arabi S. Comparison of antibacterial effects of oral rinses chlorhexidine and herbal mouth wash in patients admitted to intensive care unit. Bratisl Lek Listy. 2012; 113(9);556 [view at publisher] [DOI] [PMID] [Google Scholar]
20. Ali N.H, Faizi S, Kazmi S.U. Antibacterial activity in spices and local medicinal plants against clinical isolates of Karachi, Pakistan. Pharm Biol. 2011; 49(8), 833-839. [view at publisher] [DOI] [PMID] [Google Scholar]
21. AL-Izzy MY. Antimicrobial effects of aqueous and alcoholic extract of peganum harmala L.Seeds on two types of salivary isolated microorganisms in Al-Ramadi city. JKAU Med Sci. 2010; 17(4): 3-17. [view at publisher] [DOI] [Google Scholar]
22. Nenaah G. Antibacterial and antifungal activities of (beta)-carboline alkaloids of Peganum harmala (L) seeds and their combination effects. Fitoterapia. 2010; 81(7): 779-782. [view at publisher] [DOI] [PMID] [Google Scholar]

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2021 CC BY-NC 4.0 | Jorjani Biomedicine Journal

Designed & Developed by : Yektaweb