Stem Cell Research Center, Biomedical Research Institute, Golestan University of Medical Sciences, Gorgan, Iran; Department of Molecular Medicine, Faculty of Advanced Medical Technologies, Golestan University of Medical Sciences, Gorgan, Iran , ayyoobfarsian@yahoo.com
Abstract: (1655 Views)
Increasing evidence has shown that extracellular vesicles (EVs), such as exosomes (EXOs), have attracted considerable interest due to their unique biological functions as drug delivery vehicles, non-invasive tools for disease diagnosis, and their clinical potential, most prominently in the fields of oncology and cell-based therapy. Although significant advancements have been made in EXO isolation techniques, standardizing cell culture methods remains an important challenge that must be overcome to ensure specific, pure, and effective EXOs for diverse life science and clinical applications.
References
1. Kim T, Hong JW, Lee LP. Correction: Efficient methods of isolation and purification of extracellular vesicles. Nano Converg. 2025;12(1):64. [View at Publisher] [DOI] [PMID] [Google Scholar]
2. Safdar A, Saleem A, Tarnopolsky MA. The potential of endurance exercise-derived exosomes to treat metabolic diseases. . Nat Rev Endocrinol. 2016;12:504-17. [View at Publisher] [DOI] [PMID] [Google Scholar]
3. Murk JLAN, Humbel BM, Ziese U, Griffith JM, Posthuma G, Slot JW, et al. Endosomal Compartmentalization in Three Dimensions: Implications for Membrane Fusion. . Proc Natl Acad Sci U S A. 2003;100(23):13332-7. [View at Publisher] [DOI] [PMID] [Google Scholar]
4. Mathivanan S, Ji H, Simpson RJ. Exosomes: extracellular organelles important in intercellular communication. . J Proteomics. 2010;73(10):1907-20. [View at Publisher] [DOI] [PMID] [Google Scholar]
5. Dilsiz N. A comprehensive review on recent advances in exosome isolation and characterization: Toward clinical applications. . Transl Oncol.
2024:50:102121. [View at Publisher] [DOI] [PMID] [Google Scholar]
6. Pan BT, Johnstone RM. Fate of the Transferrin Receptor during Maturation of Sheep ReticulocytesIn Vitro: Selective Externalization of the Receptor. Cell. 1983;33(3):967-78. [View at Publisher] [DOI] [PMID] [Google Scholar]
7. Johnstone RM, Adam M, Hammond JR, Orr L, Turbide C. Vesicle Formation during Reticulocyte Maturation. Association of Plasma Membrane Activities with Released Vesicles (Exosomes). J Biol Chem. 1987;262(19):9412-20. [View at Publisher] [DOI] [PMID] [Google Scholar]
8. Zhang P, Zhou X, He M, Shang Y, Tetlow AL, Godwin AK,etal. Ultrasensitive Detection of Circulating Exosomes with a 3D-Nanopatterned Microfluidic Chip. Nat Biomed Eng. 2019;3(6):438-451. [View at Publisher] [DOI] [PMID] [Google Scholar]
9. Théry C, Zitvogel L, Amigorena S. Exosomes: composition, biogenesis and function. Nat Rev Immunol. 2002;2(8):569-79. [View at Publisher] [DOI] [PMID] [Google Scholar]
10. Van der Pol E, Böing AN, Harrison P, Sturk A, Nieuwland R. Classifcation, functions, and clinical relevance of extracellular vesicles. Pharmacol Rev. 2012;64(3):676-705. [View at Publisher] [DOI] [PMID] [Google Scholar]
11. Khoshsirat Sh, Keramatinia A, Khoramgah MS, Vafaei-Nezhad S, Niknazar S, Darabi Sh et al. Exosome therapy in spinal cord injury: a review. J Otorhinolaryngol Facial Plastic Surg. 2019;5(2):1-8. [View at Publisher] [DOI] [Google Scholar]
12. Lu M, Yuan S, Li S, 2, Li L, Liu M, Wan S. The exosome-derived biomarker in atherosclerosis and its clinical application. J Cardiovasc Transl Res. 2019;12(1):68-74. [View at Publisher] [DOI] [PMID] [Google Scholar]
13. Logozzi M, Di Raimo R, Mizzoni D, Fais S. Immunocapture-based ELISA to characterize and quantify exosomes in both cell culture supernatants and body fuids. Methods Enzymol. 2020:645:155-180. [View at Publisher] [DOI] [PMID] [Google Scholar]
14. Zhao Y, Weber SR, Lease J, Russo M, Siedlecki CA, Xu L-C, et al. Liquid Biopsy of Vitreous Reveals an Abundant Vesicle Population Consistent with the Size and Morphology of Exosomes. Transl Vis Sci Technol. 2018;7(3):6. [View at Publisher] [DOI] [PMID] [Google Scholar]
15. Mateescu B, Kowal EJ, van Balkom BWM, Bartel S, Bhattacharyya SN, Buzás EI, et al. Obstacles and Opportunities in the Functional Analysis of Extracellular Vesicle RNA - an ISEV Position Paper. J Extracell Vesicles. 2017;6:1286095. [View at Publisher] [DOI] [PMID] [Google Scholar]
16. Fallah A, Hosseinzadeh Colagar A, Khosravi A, Saeidi M. Exosomes from SHED-MSC regulate polarization and stress oxidative indexes in THP-1 derived M1 macrophages. Archives of Biochemistry and Biophysics 2024(755 ):109987. [View at Publisher] [DOI] [PMID] [Google Scholar]
17. Fallah A, Hosseinzadeh Colagar A, Khosravi A, Mohammad-Hasani A, Saeidi M. The role of natural exosomes from SHED-MSC in immunoregulation of M0/M1polarized macrophage cells. Front Immunol. 2025:16:1550280. [View at Publisher] [DOI] [PMID] [Google Scholar]
18. Mohammad-Hasani A, Mohammadi S, Saeidi M, Fallah A, Khosravi A. The secretome derived from serum-free media of SHED-MSCs can modulate THP-1-derived macrophage cells. .J Cell Mol Med. 2026;30(9):e71063. [View at Publisher] [DOI] [PMID] [Google Scholar]
19. Araldi E, Krämer-Albers E-M, Nolte-'t Hoen E, Peinado H, Psonka-Antonczyk KM, Rao P, et al. . International society for extracellular vesicles: first annual meeting, April 17-21: ISEV-2012. J Extracell Vesicles. 2012;1:19995. [View at Publisher] [DOI] [PMID] [Google Scholar]
20. Witwer KW , Buzás EI, Bemis LT, Bora A, Lässer C, Lötvall J, et al. Standardization of sample collection, isolation and analysis methods in extracellular vesicle research. J Extracell Vesicles. 2013;2:20360. [View at Publisher] [DOI] [PMID] [Google Scholar]
21. Chen J, Li P, Zhang T, Xu Z, Huang X, Wang R ,et al. Review on Strategies and Technologies for Exosome Isolation and Purification. Front Bioeng Biotechnol. 2022:9:811971. [View at Publisher] [DOI] [PMID] [Google Scholar]
22. Suresh PS, Zhang Q. Comprehensive Comparison of Methods for Isolation of Extracellular Vesicles from Human Plasma. J Proteome Res. 2025;24(6):2956-67. [View at Publisher] [DOI] [PMID] [Google Scholar]
23. Valadi H, Ekström K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO. Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Nat Cell Biol. 2007;9(6):654-9. [View at Publisher] [DOI] [PMID] [Google Scholar]
24. Montecalvo A, Shufesky WJ, Stolz DB, Sullivan MG, Wang Z, Divito SJ, et al. Exosomes as a short-range mechanism to spread alloantigen between dendritic cells during T cell allorecognition. J Immunol. 2008;180(5):3081-90 [View at Publisher] [DOI] [PMID] [Google Scholar]
25. Thery C, Amigorena S, Raposo G, Clayton A. Isolation and characterization of exosomes from cell culture supernatants and biological fluids. Curr Protoc Cell Biol. 2006:Chapter 3:Unit 3.22. [View at Publisher] [DOI] [PMID] [Google Scholar]
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