Volume 11, Issue 2 (10-2023)
Jorjani Biomed J 2023, 11(2): 5-8 |
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Heidari M, Dardmeh F, Alipour H, Azami N. Effect of carob (Ceratonia Siliqua) kibble extract on the kinematic parameters of human frozen-thawed sperm: A preliminary study. Jorjani Biomed J 2023; 11 (2) :5-8
URL: http://goums.ac.ir/jorjanijournal/article-1-938-en.html
URL: http://goums.ac.ir/jorjanijournal/article-1-938-en.html
1- Department of Biology, Faculty of Science, Islamic Azad University, Gorgan Branch, Gorgan, Iran. , heidarimahmoud@gorganiau.ac.ir
2- Laboratory of Regenerative Medicine, Department of Health Science and Technology, Aalborg University, Aalborg, Denmark
3- Department of Biology, Faculty of Science, Islamic Azad University, Gorgan Branch, Gorgan, Iran
2- Laboratory of Regenerative Medicine, Department of Health Science and Technology, Aalborg University, Aalborg, Denmark
3- Department of Biology, Faculty of Science, Islamic Azad University, Gorgan Branch, Gorgan, Iran
Abstract: (905 Views)
Background: Semen quality and related parameters correlate directly with fertilization, consequently assisting reproductive technology outcomes. Traditional studies on carob (Ceratonia siliqua) have demonstrated its effect on male fertility potential via the reductive effect on reactive oxygen species. This study aimed to investigate the effect of carob kibble extract on sperm motility.
Methods: The extract was made using acetone as a solvent, followed by vacuum evaporation and filtration. Following thawing, each of the forty human semen samples was divided into four groups and exposed to concentrations of 0.0 (control), 0.05, 0.2, and 0.8 mg/ml of the extract. Percentages of progressive motile, non-progressive motile, and immotile sperms, as well as other kinematic parameters, were assessed by computer-aided sperm analysis immediately after exposure to the concentrations (T0) and one hour later (T1). Data were analyzed by repeated measure analysis of variance and paired sample t-student tests using SPSS software. The level of p< 0.05 was considered statistically significant.
Results: No significant difference was found between groups at T0 or T1 values. However, a comparison of matched doses at T0 and T1 indicated that lower doses 0.05 and 0.2 mg/ml could significantly (p<0.05) inhibit natural decline in motility.
Conclusion: Adding lower doses of carob kibble extract on a thawing medium could have a supportive effect on sperm motility. However, adding the extract to a vitrification solution before a freezing process, as well as oral intake of the extract seems to have more efficiency than would be a subject for further studies.
Keywords: Carob, Kinematic parameters, Frozen sperm, Antioxidant
Methods: The extract was made using acetone as a solvent, followed by vacuum evaporation and filtration. Following thawing, each of the forty human semen samples was divided into four groups and exposed to concentrations of 0.0 (control), 0.05, 0.2, and 0.8 mg/ml of the extract. Percentages of progressive motile, non-progressive motile, and immotile sperms, as well as other kinematic parameters, were assessed by computer-aided sperm analysis immediately after exposure to the concentrations (T0) and one hour later (T1). Data were analyzed by repeated measure analysis of variance and paired sample t-student tests using SPSS software. The level of p< 0.05 was considered statistically significant.
Results: No significant difference was found between groups at T0 or T1 values. However, a comparison of matched doses at T0 and T1 indicated that lower doses 0.05 and 0.2 mg/ml could significantly (p<0.05) inhibit natural decline in motility.
Conclusion: Adding lower doses of carob kibble extract on a thawing medium could have a supportive effect on sperm motility. However, adding the extract to a vitrification solution before a freezing process, as well as oral intake of the extract seems to have more efficiency than would be a subject for further studies.
Keywords: Carob, Kinematic parameters, Frozen sperm, Antioxidant
Type of Article: Original article |
Subject:
Basic Medical Sciences
Received: 2022/11/6 | Accepted: 2023/06/18 | Published: 2023/07/1
Received: 2022/11/6 | Accepted: 2023/06/18 | Published: 2023/07/1
References
1. WHO. Infertility definitions and terminology. WHO [Internet]. 2016. [View at Publisher]
2. Hwang K, Walters RC, Lipshultz LI. Contemporary concepts in the evaluation and management of male infertility. Nat Rev Urol. 2011;8(2):86-94. [View at Publisher] [DOI] [PMID] [Google Scholar]
3. Sharlip ID, Jarow JP, Belker AM, Lipshultz LI, Sigman M, Thomas AJ, et al. Best practice policies for male infertility. Fertil Steril. 2002;77(5):873-82. [View at Publisher] [DOI] [PMID] [Google Scholar]
4. Kidd SA, Eskenazi B, Wyrobek AJ. Effects of male age on semen quality and fertility: A review of the literature. Fertil Steril. 2001;75(2):237-48. [View at Publisher] [DOI] [PMID] [Google Scholar]
5. Banihani SA, Alawneh RF. Human Semen Samples with High Antioxidant Reservoir May Exhibit Lower Post-Cryopreservation Recovery of Sperm Motility. Biomolecules. 2019;9(3):111. [View at Publisher] [DOI] [PMID] [Google Scholar]
6. Boitrelle F, Albert M, Theillac C, Ferfouri F, Bergere M, Vialard F, et al. Cryopreservation of human spermatozoa decreases the number of motile normal spermatozoa, induces nuclear vacuolization and chromatin decondensation. J Androl. 2012;33(6):1371-8. [View at Publisher] [DOI] [PMID] [Google Scholar]
7. Ozkavukcu S, Erdemli E, Isik A, Oztuna D, Karahuseyinoglu S. Effects of cryopreservation on sperm parameters and ultrastructural morphology of human spermatozoa. J Assist Reprod Genet. 2008;25(8):403-11. [View at Publisher] [DOI] [PMID] [Google Scholar]
8. Thomson LK, Fleming SD, Aitken RJ, De Iuliis GN, Zieschang JA, Clark AM. Cryopreservation-induced human sperm DNA damage is predominantly mediated by oxidative stress rather than apoptosis. Hum Reprod. 2009;24(9):2061-70. [View at Publisher] [DOI] [PMID] [Google Scholar]
9. Oberoi B, Kumar S, Talwar P. Study of human sperm motility post cryopreservation. Med J Armed Forces India. 2014;70(4):349-53. [View at Publisher] [DOI] [PMID] [Google Scholar]
10. Bateni Z, Azadi L, Tavalaee M, Kiani-Esfahani A, Fazilati M, Nasr-Esfahani MH. Addition of Tempol in semen cryopreservation medium improves the post-thaw sperm function. Syst Biol Reprod Med. 2014;60(4):245-50. [View at Publisher] [DOI] [PMID] [Google Scholar]
11. Minaei MB, Barbarestani M, Nekoonam S, Abdolvahabi MA, Takzare N, Asadi MH, et al. Effect of Trolox addition to cryopreservation media on human sperm motility. Iran J Reprod Med. 2012;10(2):99-104. [View at Publisher] [PMID] [Google Scholar]
12. Mollaahmadi L, Keramat A, Ghiasi A, Hashemzadeh M. The relationship between semen parameters in processed and unprocessed semen with intrauterine insemination success rates. J Turk Ger Gynecol Assoc. 2019;20(1):1-7. [View at Publisher] [DOI] [PMID] [Google Scholar]
13. Moreau J, Gatimel N, Parinaud J, Léandri RD. Does the 18-h sperm motility influence intrauterine insemination results? Andrology. 2018;6(5):805-6. [View at Publisher] [DOI] [PMID] [Google Scholar]
14. Clark NA, Will M, Moravek MB, Fisseha S. A systematic review of the evidence for complementary and alternative medicine in infertility. Int J Gynaecol Obstet. 2013;122(3):202-6. [View at Publisher] [DOI] [PMID] [Google Scholar]
15. Iii RC, Malak A, Eric L. Role of Complementary and Alternative Medicine in Male. 2018;3:1-5. [View at Publisher] [Google Scholar]
16. Batlle I, Tous J. Carob tree Ceratonia siliqua L. Promoting the conservation and use of underutilized and neglected crops. Rome: Genet Resour Institute:1997. 92p. [View at Publisher] [Google Scholar]
17. Vafaei A, Mohammadi S, Fazel A, Soukhtanloo M, Mohammadipour A, Beheshti F. Effects of Carob (Ceratonia siliqua) on Sperm Quality, Testicular Structure, Testosterone Level and Oxidative Stress in Busulfan-Induced Infertile Mice. Pharm Sci. 2018;24(2):104-11. [View at Publisher] [DOI] [Google Scholar]
18. Nasar-Abbas SM, e-Huma Z, Vu TH, Khan MK, Esbenshade H, Jayasena V. Carob Kibble: A Bioactive-Rich Food Ingredient. Compr Rev Food Sci Food Saf. 2016;15(1):63-72. [View at Publisher] [DOI] [PMID] [Google Scholar]
19. Asgari M, Khodaei Motlagh M, Kazemi Bonchenari M, Vahedi V. Antioxidant effect of carob seedextract (Ceratoniasiliqua L) on quality parameters Farahani ram sperm after freeze-thawing. J Cell Tissue. 2020;11(1):1-12. [View at Publisher] [DOI] [Google Scholar]
20. Nemati Z, Dehgani P, Besharati M, Amirdahri S. Dietary carob fruit (Ceratonia siliqua L.) supplementation improves spermatogenesis, semen quality and embryonic death via antioxidant effect in aging broiler breeder roosters. Anim Reprod Sci. 2022;239:106967. [View at Publisher] [DOI] [PMID] [Google Scholar]
21. Aghajani MMR, Mahjoub S, Mojab F, Namdari M, Gorji NM, Dashtaki A, et al. Comparison of the Effect of Ceratonia Siliqua L.(Carob) Syrup and Vitamin E on Sperm Parameters, Oxidative Stress Index, and Sex Hormones in Infertile Men: A Randomized Controlled Trial. Reprod Sci. 2021;28(3):766-74. [View at Publisher] [DOI] [PMID] [Google Scholar]
22. Soleimanzadeh A, Kian M, Moradi S, Mahmoudi S. Carob (Ceratonia siliqua L.) fruit hydro-alcoholic extract alleviates reproductive toxicity of lead in male mice: Evidence on sperm parameters, sex hormones, oxidative stress biomarkers and expression of Nrf2 and iNOS. Avicenna J phytomed. 2020;10(1):35-49. [View at Publisher] [DOI] [PMID] [Google Scholar]
23. Faramarzi A, Aghaz F, Golestan Jahromi M, Bakhtiari M, Khazaei M. Does supplementation of sperm freezing/thawing media with Ceratonia siliqua improve detrimental effect of cryopreservation on sperm parameters and chromatin quality in normozoospermic specimens?. Cell Tissue Bank. 2019;20(3):403-9. [View at Publisher] [DOI] [PMID] [Google Scholar]
24. Makris DP, Kefalas P. Carob Pods ( Ceratonia siliqua L .) as a Source of Polyphenolic Antioxidants. Food Technol Biotechnol. 2004;42(2):105-8. [View at Publisher] [Google Scholar]
25. World Health Organization. Examination and processing of human semen. 5th Ed. WHO Lab Man. 2010. [View at Publisher] [Google Scholar]
26. Reyes-San-Martin C, Hamoh T, Zhang Y, Berendse L, Klijn C, Li R, et al. Nanoscale MRI for Selective Labeling and Localized Free Radical Measurements in the Acrosomes of Single Sperm Cells. ACS Nano. 2022;16(7):10701-10. [View at Publisher] [DOI] [PMID] [Google Scholar]
27. Sati L, Huszar G. Sperm motility and viability: Overview of the cellular and physiological aspects that support these functions. Eur Med J Repro Health. 2015;1(1):74-80. [View at Publisher] [DOI] [Google Scholar]
28. Bansal AK, Bilaspuri GS. Impacts of Oxidative Stress and Antioxidants on Semen Functions. Vet Med Int. 2011;2011:1-7. [View at Publisher] [DOI] [PMID] [Google Scholar]
29. Imamovic Kumalic S, Pinter B. Review of clinical trials on effects of oral antioxidants on basic semen and other parameters in idiopathic Oligoasthenoteratozoospermia. Biomed Res Int. 2014;2014:426951. [View at Publisher] [DOI] [PMID] [Google Scholar]
30. Zhong R zhen, Zhou D wei. Oxidative stress and role of natural plant derived antioxidants in animal reproduction. J Integr Agric. 2013;12(10):1826-38. [View at Publisher] [DOI] [PMID] [Google Scholar]
31. Ferramosca A, Zara V. Diet and male fertility: The impact of nutrients and antioxidants on sperm energetic metabolism. Int J Mol Sci. 2022;23(5):2542. [View at Publisher] [DOI] [PMID] [Google Scholar]
32. Mahdiani E, Khadem Haghighian H, Javadi M, Karami AA, Kavianpour M. Effect of carob (Ceratonia siliqua L.) oral supplementation on changes of semen parameters, oxidative stress, inflammatory biomarkers and reproductive hormones in infertile men. Sci J Kurdistan Univ Med Sci. 2018;23(3):56-66. [View at Publisher] [DOI] [Google Scholar]
33. Salas-Huetos A, Bulló M, Salas-Salvadó J. Dietary patterns, foods and nutrients in male fertility parameters and fecundability: A systematic review of observational studies. Hum Reprod Update. 2017;23(4):371-89. [View at Publisher] [DOI] [PMID] [Google Scholar]
34. Abbas K, Batool U, Memon AS, Soomro S. Oxidative Stress: A Double Edged Sword. BioSight. 2021;2(1):4-12. [View at Publisher] [DOI] [PMID] [Google Scholar]
35. Ibrahim SF, Osman K, Das S, Othman AM, Majid NA, Rahman MPA. A study of the antioxidant effect of alpha lipoic acids on sperm quality. Clinics (Sao Paulo). 2008;63(4):545-50. [View at Publisher] [DOI] [PMID] [Google Scholar]
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