Volume 10, Issue 1 (3-2022)                   Jorjani Biomed J 2022, 10(1): 56-66 | Back to browse issues page

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Alemrajabi M, Sadrzadeh-Afshar M, dastorani M, Barjestehnia M. Effects of Propolis and Persica Mouthwashes on Minor Aphthous Ulcers: A Comparative Study. Jorjani Biomed J. 2022; 10 (1) :56-66
URL: http://goums.ac.ir/jorjanijournal/article-1-872-en.html
1- Assistant Professor, Oral & Maxillofacial medicine department, Faculty of Dentistry, Aja University of medical sciences, Tehran, Iran
2- Assistant professor, Oral and maxilofacial medicine department, Faculty of Dentistry, Aja university of medical sciences, Tehran, Iran , m_sadrzade@alumnus.tums.ac.ir
3- Assistant Professor ,Endodontics department, Faculty of Dentistry, Aja University of medical sciences, Tehran, Iran
4- Dentist, Oral & Maxillofacial medicine department, Faculty of Dentistry, Aja University of medical sciences, Tehran, Iran
Abstract:   (719 Views)
Background and objectives: Recurrent Aphthous Stomatitis (RAS) is a common disease of the nonkeratinized oral mucosa characterized by painful ulcerations and inflammation, causing difficulty in eating, swallowing, and speaking. Symptomatic treatment is considered for this disease due to the ambiguity of the exact etiology. The beneficial therapeutic effects of Persica and Propolis mouthwashes led us to the objective of studying therapeutic potentials of these herbal mouthwashes.
Material and Methods: The present double-blind clinical trial was conducted on 40 patients with minor aphthous ulcers and no systemic disease, who were divided into two groups of 20 via random allocation (systematic random sampling). Each group received 15 drops of propolis or persica mouthwashes three times a day for 10 days. Pain intensity was recorded by measuring the score of Visual Analogue Scale (VAS) and the diameter of minor aphthous lesions (in mm) at three periods of before treatment, and on the second and sixth days after treatment. Data were analyzed by Mann-Whitney test.
Results: There was no significant difference between pain intensity and ulcer size before treatment and on the second day of treatment. The pain intensity on day 6 was significantly lower in Persica group (P=0.045) but no difference was observed in the mean ulcer size on day 6 between the two groups (P=0.104). The rate of pain relief (P=0.031) and reduction of ulcer size (P=0.046) were significantly higher in Persica group.
Conclusion: Propolis and Persica mouthwashes both reduce pain intensity and ulcer size in minor aphthous ulcers, and Persica showed a stronger potential to achieve these goals compared to propolis.
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 Aphthous stomatitis is a multifactorial disease, the exact cause has not yet been determined.
 Many drugs are currently used to treat aphthous stomatitis, most of which are chemical and have side effects. Therefore herbal medicine can be promising.

Type of Article: Original article | Subject: General medicine
Received: 2021/12/5 | Accepted: 2022/02/27 | Published: 2022/03/30

1. Giannetti L, Murri Dello Diago A, Lo Muzio L. Recurrent aphtous stomatitis. Minerva Stomatol. 2018 Jun;67(3):125-128. [DOI] [PMID] [Google Scholar]
2. Sereflican M, Sereflican B, Dagistan E, Goksugur N, Kizildag B. Subclinical atherosclerosis in patients with recurrent aphthous stomatitis. Oral Dis. 2016 Sep;22(6):573-7. [DOI] [PMID]
3. Rajaei-Behbahani L, Abbasi F, Sadrzadeh-Afshar M-S, Rajaei-Behbahani S, Afshar S. Effects of tetracycline and Myrtus communis extract on the treatment of recurrent aphthous ulcers: A comparative study. J Bas Res Med Sci 2021; 8(2):20-26. [Google Scholar]
4. Queiroz SIML, Silva MVAD, Medeiros AMC, Oliveira PT, Gurgel BCV, Silveira ÉJDD. Recurrent aphthous ulceration: an epidemiological study of etiological factors, treatment and differential diagnosis. An Bras Dermatol. 2018;93(3):341-346. [view at publisher] [DOI] [PMID] [PMCID] [Google Scholar]
5. Gholizadeh N, Sadrzadeh-Afshar MS, Sheykhbahaei N. Intralesional corticosteroid injection as an effective treatment method for oral lesions: a metaanalysis. Braz J Pharm Sci. 2020;56:e18077. [view at publisher] [DOI] [Google Scholar]
6. Wang Z, Cao H, Xiong J, Lu Y, Deng Y, Nan H, Zheng S, Ye H, Cao Z. Recent advances in the aetiology of recurrent aphthous stomatitis (RAS). Postgrad Med J. 2022 Jan;98(1155):57-66. [view at publisher] [DOI] [PMID] [Google Scholar]
7. Wang Z, Cao H, Xiong J, Lu Y, Deng Y, Nan H, Zheng S, Ye H, Cao Z. Recent advances in the aetiology of recurrent aphthous stomatitis (RAS). Postgrad Med J. 2022 Jan;98(1155):57-66. [view at publisher] [DOI] [PMID] [Google Scholar]
8. Alemrajabi MS, Dastorani M, Hamidi H, Mohammadian S, Sadrzadeh Afshar MS. Effects of Propolis and Persica Mouthwashes on Three Common Oral Streptococci: A Comparative Study. Pharm Biomed Res. 2021; 7(4):303-316. [view at publisher] [Google Scholar]
9. Kooshki F, Tabatabaei FS, Tajik S, Aayan A. The comparison of antimicrobial effects of herbal and chemical agents on toothpaste: An experimental study. Dent Res J (Isfahan). 2018 Jul-Aug;15(4):289-294. [Google Scholar]
10. Guven Y, Ustun N, Tuna EB, Aktoren O. Antimicrobial Effect of Newly Formulated Toothpastes and a Mouthrinse on Specific Microorganisms: An In Vitro Study. Eur J Dent. 2019 May;13(2):172-177. [view at publisher] [DOI] [PMID] [PMCID] [Google Scholar]
11. Walsh T, Worthington HV, Glenny AM, Marinho VC, Jeroncic A. Fluoride toothpastes of different concentrations for preventing dental caries. Cochrane Database Syst Rev. 2019;3(3):CD007868. [view at publisher] [DOI] [PMID] [PMCID] [Google Scholar]
12. Przybyłek I, Karpiński TM. Antibacterial Properties of Propolis. Molecules. 2019 May 29;24(11):2047. [view at publisher] [DOI] [PMID] [PMCID] [Google Scholar]
13. Jabbarifar SE, Tabibian SA, Poursina F. Effect of Fluoride Mouthrinse and Toothpaste on Number of Streptococcal Colony Forming Units of Dental Plaque. J Res Med Sci 2005; 10(6): 363-7. [view at publisher] [Google Scholar]
14. Sforcin JM. Biological Properties and Therapeutic Applications of Propolis. Phytother Res. 2016 Jun;30(6):894-905. [view at publisher] [DOI] [PMID] [Google Scholar]
15. Havsteen BH. The biochemistry and medical significance of the flavonoids. Pharmacol Ther. 2002 Nov-Dec;96(2-3):67-202. [view at publisher] [DOI] [Google Scholar]
16. S.Hariri, A., Shayesteh, S., Asgharian, P., Chamanara, M., & Sadrzadeh-Afshar, M-S. Eremostachys Binalodensis, A Potential Therapeutic Choice for Gingival Inflammatory Wounds. J Contemp Med Sci. 2021; 7(3): 140-144. [DOI]
17. Eming SA, Martin P, Tomic-Canic M. Wound repair and regeneration: mechanisms, signaling, and translation. Sci Transl Med. 2014 Dec 3;6(265):265sr6. [view at publisher] [DOI] [PMID] [PMCID] [Google Scholar]
18. Delgado DA, Lambert BS, Boutris N, McCulloch PC, Robbins AB, Moreno MR, Harris JD. Validation of Digital Visual Analog Scale Pain Scoring With a Traditional Paper-based Visual Analog Scale in Adults. J Am Acad Orthop Surg Glob Res Rev. 2018 Mar 23;2(3):e088. [DOI] [PMID] [PMCID] [Google Scholar]
19. Darbandi A, Nikfar F. Comparison between the two mouth rinses (Persica & antiseptic Irsha) on recurrent aphthous stomatitis. J Dent Sch. 2007; 24 (4):435-438. [Google Scholar]
20. Braakhuis A. Evidence on the Health Benefits of Supplemental Propolis. Nutrients. 2019 Nov 8;11(11):2705. [view at publisher] [DOI] [PMID] [PMCID] [Google Scholar]
21. Mungmunpuntipantip R, Wiwanitkit V. Vulvar Aphthous Ulcer and COVID-19 mRNA Vaccination. J Pediatr Adolesc Gynecol. 2021 Dec 31:S1083-3188(21)00361-2. [view at publisher] [Google Scholar]
22. Koo H, Rosalen PL, Cury JA, Park YK, Bowen WH. Effects of compounds found in propolis on Streptococcus mutans growth and on glucosyltransferase activity. Antimicrob Agents Chemother. 2002 May; 46(5):1302-9. [view at publisher] [DOI] [PMID] [PMCID] [Google Scholar]
23. Khayyal MT, el-Ghazaly MA, el-Khatib AS, Hatem AM, de Vries PJ, el-Shafei S, Khattab MM. A clinical pharmacological study of the potential beneficial effects of a propolis food product as an adjuvant in asthmatic patients. Fundam Clin Pharmacol. 2003 Feb; 17(1):93-102. [view at publisher] [DOI] [PMID] [Google Scholar]
24. Middleton E Jr, Kandaswami C, Theoharides TC. The effects of plant flavonoids on mammalian cells: implications for inflammation, heart disease, and cancer. Pharmacol Rev. 2000; 52(4):673-751. [view at publisher] [Google Scholar]
25. Shoae Hassani AR, Hamdi K, Ghaemi A. in vitro reduction in colonization of streptococcus mutants by honey Beeswax Ethyl Acetate extract. Arak medical university Journal (Rahavard danesh ) 2009; 11 (4):87-95. [view at publisher] [Google Scholar]
26. Niedzielska I, Puszczewicz Z, Mertas A, Niedzielski D, Różanowski B, Baron S, et al. The Influence of Ethanolic Extract of Brazilian Green Propolis Gel on Hygiene and Oral Microbiota in Patients after Mandible Fractures. Biomed Res Int 2016; 2016:9190814. [view at publisher] [DOI] [PMID] [PMCID] [Google Scholar]
27. Momen-Beitollahi J, Mansorian A, Esmaili M, Amanlou M, Mohamadnia A, Bahrami N. Antimicrobial effects of propolis extract on the most prevalent oral pathogens: An in vitro study. J Islam Dent Assoc IRAN (JIDA). 2009; 21 (1): 33-39. [Google Scholar]
28. Akhavan Karbasi, MH, Forat Yazdi M, Ahadian H, Alili Sadrabad M. Evaluating Baremoom Mouthwash Efficacy in Treatment of Chemotherapy-Induced Mucositis. J Shahid Sadoughi Univ Med Sci 2016; 23(12): 1202-14. [view at publisher] [Google Scholar]
29. Samet N, Laurent C, Susarla SM, Samet-Rubinsteen N. The effect of bee propolis on recurrent aphthous stomatitis: a pilot study. Clin Oral Investig. 2007 Jun; 11(2):143-7. [view at publisher] [DOI] [PMID] [Google Scholar]
30. La Casa C, Villegas I, Alarcón de la Lastra C, Motilva V, Martín Calero MJ. Evidence for protective and antioxidant properties of rutin, a natural flavone, against ethanol induced gastric lesions. J Ethnopharmacol. 2000 Jul; 71(1-2):45-53. [view at publisher] [DOI] [Google Scholar]
31. Boyanova L, Derejian S, Koumanova R, Katsarov N, Gergova G, Mitov I, Nikolov R, Krastev Z. Inhibition of Helicobacter pylori growth in vitro by Bulgarian propolis: preliminary report. J Med Microbiol. 2003 May;52(Pt 5):417-9. [view at publisher] [DOI] [PMID] [Google Scholar]
32. Sadrzadeh-Afshar M-S, Tavafi H, Niroomand S. In Vitro Effectiveness of Antimicrobial Properties of Propolis and Chlorhexidine on Oral Pathogens: A Comparative Study. Biosis: Biological Systems .2020; 1(3):116-125. [view at publisher] [DOI] [Google Scholar]
33. Abbasi F,Haghgoo R. Effect of Persica and Irsha mouth rinses on pH of saliva. Daneshvar Med. 2011; 93(7):79-84. [view at publisher] [Google Scholar]
34. Lewis DA, Shaw GP. A natural flavonoid and synthetic analogues protect the gastric mucosa from aspirin-induced erosions. J Nutr Biochem. 2001; 12(2):95-100. [view at publisher] [DOI] [Google Scholar]
35. Arshad H, Sami MA, Sadaf S, Hassan U. Salvadora persica mediated synthesis of silver nanoparticles and their antimicrobial efficacy. Sci Rep. 2021 Mar 16;11(1):5996. [view at publisher] [DOI] [PMID] [PMCID] [Google Scholar]
36. Sforcin JM. Propolis and the immune system: a review. J Ethnopharmacol 2007; 113(1): 1-14. [view at publisher] [DOI] [PMID] [Google Scholar]
37. Šuran J, Cepanec I, Mašek T, Radić B, Radić S, Tlak Gajger I, Vlainić J. Propolis Extract and Its Bioactive Compounds-From Traditional to Modern Extraction Technologies. Molecules. 2021 May 14;26(10):2930. [view at publisher] [DOI] [PMID] [PMCID] [Google Scholar]

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