Volume 9, Issue 1 (3-2021)
Jorjani Biomed J 2021, 9(1): 16-23 |
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Karimi Fard M, Khajehlandi A. The Effect of Training at Different Temperatures on The Gene Expression of GLUT4 and Insulin Receptor in the Brown Adipose Tissue of Streptozotocin-Induced Diabetic Rats. Jorjani Biomed J 2021; 9 (1) :16-23
URL: http://goums.ac.ir/jorjanijournal/article-1-780-en.html
URL: http://goums.ac.ir/jorjanijournal/article-1-780-en.html
1- Department of Physical Education and Sport Sciences, Gachsaran Branch, Islamic Azad University, Gachsaran, Iran
2- Department of Physical Education and Sport Sciences, Gachsaran Branch, Islamic Azad University, Gachsaran, Iran ,A.khajehlandi@yahoo.com
2- Department of Physical Education and Sport Sciences, Gachsaran Branch, Islamic Azad University, Gachsaran, Iran ,
Abstract: (6057 Views)
Background and Objective: Diabetes is one of the health problems in all societies. The aim of the present study was to investigate the effect of a period of training at different temperatures on the gene expression of GLUT_4 and insulin receptor in the brown adipose tissue of diabetic rats.
Material and Methods: In this experimental study, 21 diabetic rats were randomly divided into 3 groups of 7 animals, including: (1) control (C), (2) swimming training at 5° C (S5ºC), and (3) swimming training at 36° C (S36ºC). Water swimming training was performed at 5±2° C and 36±2° C for six weeks, 5 sessions per week and 2-4 minutes per session. Data were analyzed using one-way analysis of variance and Tukey’s post hoc test at the significance level of p≤0.05.
Results: The gene expression of GLUT4 and insulin receptor in the S5º C and S36º C groups was significantly (P = 0.0001) higher than the control group. Also, the gene expression of GLUT4 in the S36º C group was higher than the S5º C group (p = 0.001), and the expression of insulin receptor in the S5º C group was significantly (p = 0.001) higher than the S36º C group.
Conclusion: Swimming training at 5º C and 36º C significantly increased the gene expression of GLUT4 and insulin receptor in the brown adipose tissue of diabetic rats.
Material and Methods: In this experimental study, 21 diabetic rats were randomly divided into 3 groups of 7 animals, including: (1) control (C), (2) swimming training at 5° C (S5ºC), and (3) swimming training at 36° C (S36ºC). Water swimming training was performed at 5±2° C and 36±2° C for six weeks, 5 sessions per week and 2-4 minutes per session. Data were analyzed using one-way analysis of variance and Tukey’s post hoc test at the significance level of p≤0.05.
Results: The gene expression of GLUT4 and insulin receptor in the S5º C and S36º C groups was significantly (P = 0.0001) higher than the control group. Also, the gene expression of GLUT4 in the S36º C group was higher than the S5º C group (p = 0.001), and the expression of insulin receptor in the S5º C group was significantly (p = 0.001) higher than the S36º C group.
Conclusion: Swimming training at 5º C and 36º C significantly increased the gene expression of GLUT4 and insulin receptor in the brown adipose tissue of diabetic rats.
Keywords: Physical Education and Training [MeSH], GLUT4 [MeSH], Insulin Receptor [MeSH], Diabetes [MeSH]
Type of Article: Original article |
Subject:
Health
Received: 2021/01/5 | Accepted: 2021/02/21 | Published: 2021/03/30
Received: 2021/01/5 | Accepted: 2021/02/21 | Published: 2021/03/30
References
1. Coskun O, Ocakci A, Bayraktaroglu T, Kanter M. Exercise training prevents and protects streptozotocininduced oxidative stress and beta-cell damage in rat pancreas. Tohoku J Exp Med 2004; 203: 145-54. [view at publisher] [DOI] [Google Scholar]
2. Greenberg AS,Mc Daniel ML. Identifying the links between obesity, insulin resistance and β‐cell function: potential role of adipocyte‐derived cytokines in the pathogenesis of type 2 diabetes. European journal of clinical investigation, 2002, 32: 24-34. [view at publisher] [DOI] [Google Scholar]
3. Handschin C, Spiegelman BM. The role of exercise and PGC1α in inflammation and chronic disease. Nature. 2008 Jul;454(7203):463-9. [view at publisher] [DOI] [Google Scholar]
4. Kajimura S, Saito M. A new era in brown adipose tissue biology: molecular control of brown fat development and energy homeostasis. Annu Rev Physiol 2014;76:225. [view at publisher] [DOI] [Google Scholar]
5. Nedergaard, J.; Golozoubova, V.; Matthias, A.; Asadi, A.; Jacobsson, A.; Cannon, B. UCP1: The only protein able to mediate adaptive non-shivering thermogenesis and metabolic inefficiency. Biochim. Biophys. Acta 2001, 1504, 82-106. [view at publisher] [DOI] [Google Scholar]
6. Hanssen MJW, Hoeks J, Brans B, Van Der Lans AAJJ, Schaart G, Van Den Driessche JJ, et al. Short-term cold acclimation improves insulin sensitivity in patients with type 2 diabetes mellitus. Nat Med. 2015; 21 (8): 863. [view at publisher] [DOI] [Google Scholar]
7. Mohebbi H, Rohani H, Hassan-Nia S. The effect of 12 weeks endurance training at 2 different intensities on GLUT4 mRNA expression of soleus and gastrocnemius muscles in obese mice. Apunts Medicina de l" Esport (English Edition). 2016; 51(191):93-9. [view at publisher] [DOI] [Google Scholar]
8. Kim BH, Newton RA, Sachs ML, Glutting JJ, Glanz K. Effect of Guided Relaxation and Imagery on Falls Self Efficacy: A Randomized Controlled Trial. J Am Geriatr Soc. 2012; 60(6):1109-14. [view at publisher] [DOI] [Google Scholar]
9. Augustin R. The protein family of glucose transport facilitators: It's not only about glucose after all. IUBMB life. 2010; 62(5):315-33. [view at publisher] [DOI] [Google Scholar]
10. Lehnen AM, Leguisamo NM, Pinto GH, Markoski MM, Angelis K, Machado UF, et al. The beneficial effects of exercise in rodents are preserved after detraining a phenomenon unrelated to GLUT4 expression. Cardiovasc Diabetol2010;9:67. [view at publisher] [DOI] [Google Scholar]
11. Gurley JM, Griesel BA, Olson AL. Increased skeletal muscle GLUT4 expression in obese Mice after voluntary wheel running exercise is posttranscriptional. Diabetes 2016;65:2911- 9. [view at publisher] [DOI] [Google Scholar]
12. Hou C-W, Chou S-W, Ho H-Y, Lee W-C, Lin C-H, Kuo C-H. Interactive effect of exercise training and growth hormone administration on glucose tolerance and muscle GLUT4 protein expression in rats. Journal of biomedical science. 2003; 10(6):689-96. [view at publisher] [DOI] [Google Scholar]
13. Goodyear LJ, Kahn BB. Exercise, glucose transport, and insulin sensitivity. Annual review of medicine. 1998; 49(1):235-61. [view at publisher] [DOI] [Google Scholar]
14. Lubkowska A, Bryczkowska I, Gutowska I, Rotter I, Marczuk N, Baranowska-Bosiacka I, et al. The effects of swimming training in cold water on antioxidant enzyme activity and lipid peroxidation in erythrocytes of male and female aged rats. Int J Environ Res Public Health. 2019; 16 (4): 647. [view at publisher] [DOI] [Google Scholar]
15. Bryczkowska I, Baranowska-Bosiacka I, Lubkowska A. Effect of repeated cold water swimming exercise on adaptive changes in body weight in older rats. Cent Eur J Sport Sci Med. 2017; 18 (2): 77- 87. [view at publisher] [DOI] [Google Scholar]
16. Jorge M.L.M.P.," et al", The effects of aerobic, resistance, and combined exercise on metabolic control, inflammatory markers, adipocytokines and muscle insulin signaling in patients with type 2 diabetes mellitus, Metabolism, 2011; 60(9): p. 1244-1252. [view at publisher] [DOI] [Google Scholar]
17. Hussey S, Mcgee S, Garnham A, Wentworth J, Jeukendrup A, Hargreaves M. Exercise training increases adipose tissue GLUT4 expression in patients with type 2 diabetes. Diabetes Obes Metab 2011;13:959-62. [view at publisher] [DOI] [Google Scholar]
18. Zarekar M, Saghebjoo M, Foadodini M, Hedayati M. Combined effect of aerobic raining and pistacia athlantica extract on GLUT-4 protein expression and muscle glycogen in diabetic rats. Iranian Journal of Endocrinology and Metabolism. 2014; 16(4):245-53. [in Persian]. [Google Scholar]
19. Ihsan M, Watson G, Abbiss CR. PGC-1α mediated muscle aerobic adaptations to exercise, heat and cold exposure. J Cell Mol Exer Physiol. 2014; 3(1):e7. [view at publisher] [DOI] [Google Scholar]
20. Richter EA, Hargreaves M. Exercise, GLUT4, and skeletal muscle glucose uptake. Physiological reviews. 2013; 93(3):993-1017. [view at publisher] [DOI] [Google Scholar]
21. Smith JA, Kohn TA, Chetty AK, Ojuka EO. CaMK activation during exercise is required for histone hyperacetylation and MEF2A binding at the MEF2 site on the Glut4 gene.American Journal of Physiology- Endocrinology and Metabolism2008;295(3): E698-E704. [view at publisher] [DOI] [Google Scholar]
22. Lim S, Choi SH, Jeong IK, Kim JH, Moon MK, Park KS, Lee HK, Kim YB, Jang HC. Insulin-sensitizing effects of exercise on adiponectin and retinol-binding protein-4 concentrations in young and middle-aged women. The Journal of Clinical Endocrinology & Metabolism. 2008 Jun 1;93(6):2263-8. [view at publisher] [DOI] [Google Scholar]
23. American Diabetes Association. Standards of medical care in diabetes-2015: summary of revisions. Diabetes Care, 2015, 38: S4. [DOI] [Google Scholar]
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