Volume 8, Issue 3 (10-2020)                   Jorjani Biomed J 2020, 8(3): 27-35 | Back to browse issues page

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Onsori H, Poladi D, Valizadeh M, Fathi A, Damandan M, Moradpour R. Molecular Identification of G6PD Chatham (1003 G>A) in North-West Iran. Jorjani Biomed J. 2020; 8 (3) :27-35
URL: http://goums.ac.ir/jorjanijournal/article-1-743-en.html
1- Cellular and Molecular Biology Department, Marand Branch, Islamic Azad University, Marand, Iran
2- Department Genetics, Ahar Branch, Islamic Azad University, Ahar, Iran
3- Unit of Genomics Research, Digestive Diseases Research Center, Ardabil University of Medical Sciences, Ardabil, Iran , mehdi_valizadeh65@yahoo.com
4- Pediatric Hematology & Oncology Department, Ardabil University of Medical Sciences, Ardabil, Iran
5- Center for Cell Pathology Research, Department of Life Science, Khazar University, Baku, Azerbaijan/Cellular and Molecular Research Center, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran
6- Cellular and Molecular Research Center, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran/Center for Cell Pathology Research, Department of Life Science, Khazar University, Baku, Azerbaijan
Abstract:   (437 Views)
Background and objective: Glucose 6-phosphate dehydrogenase (G6PD) deficiency is one of the most common human diseases with approximately 400 million people affected worldwide. G6PD Chatham is caused by 1003 G>A mutation leads to a severe enzymatic deficiency. The aim of the present study is to investigate the frequency rate of the Chatham mutations in the population of the North-West of Iran.
Material And Method: In this study, by Rapid Genomic DNA Extraction (RGDE) method, from 90 peripheral blood samples of unrelated male and female patients with genetic deficiency of G6PD, DNA was extracted and after digestion by Bstx1 enzymes, in order to search for Chatham mutation, they were analyzed by means of PCR-RFLP and sequencing methods.
Result: According to the results, Chatham mutation was observed in 10 samples (11.11%).
Conclusion: This study showed that G6PD Chatham (1003 G>A) mutation is the second common mutation, after Mediterranean (563C>T), in the population of the North-West of Iran. Further studies are recommended to identify the mutation type of other varieties.
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Type of Article: Original article | Subject: Molecular Sciences
Received: 2020/07/16 | Accepted: 2020/08/3 | Published: 2020/10/1

1. Khodashenas E, Kalani-Moghaddam F, Araghi Z, Khodaparast, M, Yazdani, Z. Glucose-6-Phosphate Dehydrogenase Deficiency and Neonatal Hyperbilirubinemia. Iranian Journal of Neonatology 2015; 6(3): 28-31.doi: 10.22038/ijn.2015.4897. [Google Scholar]
2. Lee J, Kim TI, Kang J, et al. Prevalence of glucose-6-phosphate dehydrogenase (G6PD) deficiency among malaria patients in Upper Myanmar. BMC Infect Dis. 2018; 18, 131. https://doi.org/10.1186/s12879-018-3031-y [view at publisher] [DOI] [Google Scholar]
3. Lo E, Zhong, D, Raya B. et al. Prevalence and distribution of G6PD deficiency: implication for the use of primaquine in malaria treatment in Ethiopia. Malar J. 2019; 18, 340. https://doi.org/10.1186/s12936-019-2981-x [view at publisher] [DOI] [Google Scholar]
4. Mehta A, Mason P, and Valliamy TJ. Glocuse-6-phosphate dehydrogenase deficiency. Bailliers Clinical Hematology. 2002;13: 21-38. [view at publisher] [DOI] [Google Scholar]
5. Beutler E. Molecular heterogeneity of glucose-6-phasphate dehydrogenase. Blood. 1989; 74: 2550. [DOI] [Google Scholar]
6. Haung Ch.-Sh. Neonatal jaundice and molecular mutation in G6PD deficient newborn infants. American Journal of Hematology.1995; 51: 19-25. https://doi.org/10.1002/(SICI)1096-8652(199601)51:1<19::AID-AJH4>3.0.CO;2-A [view at publisher] [DOI] [Google Scholar]
7. Noori-Daloii MR. A comprehensive study on the major mutations in G6PD deficient polymorphic variants identified in the coastal provinces of Caspian Sea in the north of Iran. Clin Biochem. 2007; 40: 699-704. [view at publisher] [DOI] [Google Scholar]
8. Valizadeh M, Onsori H, Fathi A, Bonyadi MJ, Rezamand A, Amani F. Molecular Identification of Mediterranean Mutation in Patients with Deficiency of Glucose-6-Phosphate Dehydrogenase (G6pd) in North West of Iran. J Phys Pharm Adv. 2014; 4(7): 389-395. [DOI] [Google Scholar]
9. Saremi Mohammad Ali et al. Rapid genomic DNA extraction (RGDE). Protocol Online PID. 2010; 4791, http://www.protocol-online.org [Google Scholar]
10. Noori Daloii MR, Najafi L, Mohammad Ganji S, Hajebrahimi Z, Sanati MH. Molecular identification of mutations in G6PD gene in patients with favism in Iran. J Physol Biochem.2004; 60(4); 273-7. [view at publisher] [DOI] [Google Scholar]
11. WHO Working Group. World map of G6PD deficiency. Bull WHO.1989; 67: 601-611. [Google Scholar]
12. Moosazadeh M, Nekoei-Moghadam M, Aliramzany M, Amiresmaili M. Identification of Mutation of Glucose-6-Phosphate Dehydrogenase (G6PD) in Iran: Meta- analysis Study. Iranian J Publ Health. 2013;42( 9): 1007-1015. [view at publisher] [Google Scholar]
13. Altay C, Gümrük F. Red cell glucose-6-phosphate dehydrogenase deficiency in Turkey. Turkish Journal of Haematology : Official Journal of Turkish Society of Haematology. 2008 Mar;25(1):1-7. [view at publisher] [Google Scholar]
14. Al-Allawi N, Eissa AA, Jubrael JM, Jamal SA, Hamamy H. Prevalence and molecular characterization of Glucose-6-Phosphate dehydrogenase deficient variants among the Kurdish population of Northern Iraq. BMC Blood Disord. 2010 Jul 5;10:6. doi: 10.1186/1471-2326-10-6. PMID: 20602793; PMCID: PMC2913952. [view at publisher] [DOI] [Google Scholar]
15. Carmencita P, Cutiongco E M, Silao L. Characterization of Mutations and Polymorphisms in the G6PD Gene among Filipino Newborns with G6PD Deficiency Acta medica Philippina.2011 45(4):53-57. [Google Scholar]
16. Tantular IS, Matsuoka H, Kasahara Y, Pusarawati S, Kanbe T, Tuda JS, Kido Y, Dachlan YP, Kawamoto F. Incidence and mutation analysis of glucose-6-phosphate dehydrogenase deficiency in eastern Indonesian populations. Acta Med Okayama. 2010 Dec;64(6):367-73. doi: 10.18926/AMO/41322. PMID: 21173806. [Google Scholar]
17. Mesbah Namin SA, Sanati MH, Mowjoodi A, Mason PJ, Vulliamy TJ, Noori Daloii MR. Three major glucose-6-phosphate dehydrogenase-deficient polymorphic variants identified in Mazandaran state of Iran. Br J Haematol. 2002; 117(3):763-4. [view at publisher] [DOI] [Google Scholar]
18. Mortazavi Y, Mirzamohammadi F, Teremahi Ardestani M, Mirimoghadam E, Vulliamy TJ. Glucose 6-phosphate dehydrogenase deficiency in Tehran, Zanjan and Sistan- Balouchestan provinces: prevalence and frequency of Mediterranean variant of G6PD. Iran J Biotechnol. 2010; 8(4):229-233. [view at publisher] [Google Scholar]
19. Noori-Daloii MR, Hajebrahimi Z, Najafi L. Molecular identification of the most prevalent mutation of glucose-6-phosphate dehydrogenase gene in deficient patients in Gilan province. J Sci IR Iran. 2003; 14(4): 327-31. [Google Scholar]
20. Noori-Daloii MR, Hejazi, SH, Yousefi A, Mohammad ganji S, Soltani S, Javadi KR, et al. Identification of Mutations in G6PD Gene in Patients in Hormozgan Province of Iran. J Sci I R Iran. 2006; 17(4): 313-316. [view at publisher] [Google Scholar]
21. Noori-Daloii MR, Soltanian S, Mohammad Gangi SH, Yousefi A, Hejazi S, Banihashem A, et al. Molecular Identification of the Most Prevalent Mutations of Glucose-6-Phosphate Dehydrogenase (G6PD) Gene in deficient Patients in Khorasan Province of Iran. J Sci I R Iran. 2006; 17(2):103-106. [Google Scholar]
22. Gandomani MG, Khatami SR, Kazeminezhad SR, Daneshmand S, Mashayekhi A. Molecular Identification of G6PD Chatham (G1003A) in Khuzestan Province of Iran. Journal of Genetics. 2011; 90(1):143-145. [DOI] [Google Scholar]
23. Noori-Daloii MR, Yousefi A, Mohammad Ganji S, Hejazi S, Soltanian S, Sanei Moghadam E, Bozorgzade P, Sanati MH. Molecular identification of the most prevalent mutations of g6pd gene in deficient patients in Sistan and Baluchestan province of Iran. J Sci IR Iran. 2005; 16(4): 321-2. [view at publisher] [Google Scholar]
24. Kazemi Nezhad SR, Mashayekhi A, Khatami SR, Daneshmand S, Fahmi F, Ghaderigandmani M, et al. Prevalence and Molecular Identification of Mediterranean Glucose-6-Phosphate Dehydrogenase Deficiency in Khuzestan Province, Iran. Iranian J Publ Health. 2009; 38(3):127.] [Google Scholar]

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