Volume 7, Issue 4 (12-2019)                   Jorjani Biomed J 2019, 7(4): 4-10 | Back to browse issues page


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Mahmoudi S, Nasiri R, Jafari Sales A. In-vitro antibacterial effects of methanolic extract of peppermint (Mentha Piperita Lamiaceae) on standard Staphylococcus aureus, Bacillus cereus, Escherichia coli and Pseudomonas aeruginosa strain. Jorjani Biomed J. 2019; 7 (4) :4-10
URL: http://goums.ac.ir/jorjanijournal/article-1-700-en.html
1- Department of Microbial Biotechnology, School of Biological Sciences, Islamic Azad University Tehran North Branch, Tehran, Iran
2- Iran National Elite Foundation, Tehran, Iran.
3- Department of Microbiology School of Basic Sciences, Kazerun Branch, Islamic Azad University, Kazerun, Iran. , jafari_1392@yahoo.com
Abstract:   (909 Views)
Background and objectives: Microbial resistance to antibiotics is one of the most common problems in the health care system. Therefore, many efforts have been performed to find new compounds as antimicrobial compounds. This study carried out to investigate the in-vitro antibacterial effect of methanolic extract of peppermint on standard Staphylococcus aureus, Bacillus cereus, Escherichia coli and Pseudomonas aeruginosa strain.
Methods: In this experimental laboratory study, after collecting and performing pharmacognosy evaluations, methanolic extract of the peppermint plant was prepared and its antimicrobial effects on several bacteria were determined at concentrations of 20 to 400 mg/ml using the agar well diffusion method, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC)  methods. The test was repeated five times for each bacterium and the collected data were analyzed using SPSS software.
Results: It was revealed that the methanolic extract of peppermint had antimicrobial effects against standard Staphylococcus aureus, Bacillus cereus, Escherichia coli and Pseudomonas aeruginosa. The highest effect was observed in S. aureus and the least in P. aeruginosa.  MIC and MBC of methanol extract on S. aureus, B. cereus, E. coli, and P. aeruginosa were 6.5-15.5, 15.5-25, 50-100, and 100-200 mg/ml, respectively.
Conclusion: The results of this study reveal that peppermint can be considered as an antibacterial medicinal herb and that if the concentration of the extract is higher, its antibacterial properties will also increase. Thus, it can be used as an alternative to the usual chemical drugs in the treatment of infections after evaluating their effects in vitro.
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Type of Article: Original article | Subject: Basic Medical Sciences
Received: 2019/05/10 | Revised: 2020/10/19 | Accepted: 2019/09/10

References
1. Ahameethunisa AR, Hopper W. In vitro antimicrobial activity on clinical microbial strains and antioxidant properties of Artemisia parviflora. Ann Clin Microbiol Antimicrob. 2012;11(1):30. [DOI] [Google Scholar]
2. Hancock REW. Mechanisms of action of newer antibiotics for Gram-positive pathogens. Lancet Infect Dis. 2005;5(4):209-18. [DOI] [Google Scholar]
3. Uniyal SK, Singh KN, Jamwal P, Lal B. Traditional use of medicinal plants among the tribal communities of Chhota Bhangal, Western Himalaya. J Ethnobiol Ethnomed. 2006;2(1):14. [DOI] [Google Scholar]
4. Shah PM. The need for new therapeutic agents: what is in the pipeline? Clin Microbiol Infect. 2005;11:36-42. [DOI] [Google Scholar]
5. Rojas JJ, Ochoa VJ, Ocampo SA, Muñoz JF. Screening for antimicrobial activity of ten medicinal plants used in Colombian folkloric medicine: A possible alternative in the treatment of non-nosocomial infections. BMC Complement Altern Med. 2006;6(1):1-6. [DOI] [Google Scholar]
6. Djeussi DE, Noumedem JAK, Seukep JA, Fankam AG, Voukeng IK, Tankeo SB, et al. Antibacterial activities of selected edible plants extracts against multidrug-resistant Gram-negative bacteria. BMC Complement Altern Med. 2013;13(1):164. [DOI] [Google Scholar]
7. Sagdic O, Karahan AG, Ozcan M, Ozkan G. Note: effect of some spice extracts on bacterial inhibition. Food Sci Technol Int. 2003;9(5):353-8. [DOI] [Google Scholar]
8. Thorne RF. Classification and geography of the flowering plants. Bot Rev. 1992;58(3):225-327. [DOI] [Google Scholar]
9. Wichtl M. Herbal drugs and phytopharmaceuticals: a handbook for practice on a scientific basis. Medpharm GmbH Scientific Publishers; 2004. [Google Scholar]
10. Kumar P, Mishra S, Malik A, Satya S. Insecticidal properties of Mentha species: a review. Ind Crops Prod. 2011;34(1):802-17. [DOI] [Google Scholar]
11. Sattari M, Shahbazi N, Najar Peeryeh S. An assessment of antibacterial effect of alcoholic and aquatic extracts of Eucalyptus leaves on Pseudomonas aeruginosa. Pathobiol Res. 2006;8(1):19-23. [Google Scholar]
12. Halliwell B, Gutteridge JMC. Free radicals in biology and medicine. Oxford University Press, USA; 2015. [DOI] [Google Scholar]
13. Ames BN. Dietary carcinogens and anticarcinogens: oxygen radicals and degenerative diseases. Science (80- ). 1983;221(4617):1256-64. [DOI] [Google Scholar]
14. Tylor VE. The Honest Herbal. Binghamton: Pharmaceatrial Products Press; 1993. [Google Scholar]
15. Iscan G, Kirimer R, Kurckuoglu M. Hunsu Can Baser K., Demirci F.: Screening of Mentha piperita essential oils. J Agric Food Chem. 2002;50:3943-6. [DOI] [Google Scholar]
16. Dorman HJD, Koşar M, Başer KHC, Hiltunen R. Phenolic profile and antioxidant evaluation of Mentha x piperita L.(peppermint) extracts. Nat Prod Commun. 2009;4(4):1934578X0900400419. [DOI] [Google Scholar]
17. Moreira MR, Ponce AG, Del Valle CE, Roura SI. Inhibitory parameters of essential oils to reduce a foodborne pathogen. LWT-Food Sci Technol. 2005;38(5):565-70. [DOI] [Google Scholar]
18. Rasooli I, Gachkar L, Yadegarinia D, Bagher Rezaei M, Alipoor Astaneh S. Antibacterial and antioxidative characterisation of essential oils from Mentha piperita and Mentha spicata grown in Iran. Acta Aliment. 2008;37(1):41-52. [DOI] [Google Scholar]
19. Kaur J, Rathinam X, Kasi M, Leng KM, Ayyalu R, Kathiresan S, et al. Preliminary investigation on the antibacterial activity of mango (Mangifera indica L: Anacardiaceae) seed kernel. Asian Pac J Trop Med. 2010;3(9):707-10. [DOI] [Google Scholar]
20. Singh R, Shushni MAM, Belkheir A. Antibacterial and antioxidant activities of Mentha piperita L. Arab J Chem. 2015;8(3):322-8. [DOI] [Google Scholar]
21. Pramila DM, Xavier R, Marimuthu K, Kathiresan S, Khoo ML, Senthilkumar M, et al. Phytochemical analysis and antimicrobial potential of methanolic leaf extract of peppermint (Mentha piperita: Lamiaceae). J Med Plants Res. 2012;6(2):331-5. [DOI] [Google Scholar]
22. Beuchat LR, Golden DA. Antimicrobials occurring naturally in foods. Food Technol. 1989; [Google Scholar]
23. Zaidi S, Dahiya P. In vitro antimicrobial activity, phytochemical analysis and total phenolic content of essential oil from Mentha spicata and Mentha piperita. Int Food Res J. 2015;22(6):2440. [Google Scholar]

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