Volume 7, Issue 3 (9-2019)
Jorjani Biomed J 2019, 7(3): 34-44 |
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sayyahi J, Mobaiyen H, Jafari B, Jafari-Sales A. Antibacterial effects of methanolic extracts of Reum ribes L. and Hyssopus officinalis on some standard pathogenic bacteria. Jorjani Biomed J 2019; 7 (3) :34-44
URL: http://goums.ac.ir/jorjanijournal/article-1-672-en.html
URL: http://goums.ac.ir/jorjanijournal/article-1-672-en.html
1- Young Researchers and Elite Club, Ahar Branch, Islamic Azad University, Ahar, Iran , taranoom.isi.isc@gmail.com
2- Department of Microbiology, Tabriz branch, Islamic Azad University, Tabriz, Iran
3- Department of Microbiology, Ahar Branch, Islamic Azad University, Ahar, Iran
4- Young Researchers and Elite Club, Ahar Branch, Islamic Azad University, Ahar, Iran
2- Department of Microbiology, Tabriz branch, Islamic Azad University, Tabriz, Iran
3- Department of Microbiology, Ahar Branch, Islamic Azad University, Ahar, Iran
4- Young Researchers and Elite Club, Ahar Branch, Islamic Azad University, Ahar, Iran
Abstract: (7860 Views)
Background and objectives: As much as people become aware of the dangerous side effects of synthetic antibiotics, the demand for natural alternatives to these drugs increases. Natural ingredients, lower risk of complications and even have beneficial side effects. The aim of this study was to determine the antibacterial effect of herbs Reum ribes L and hyssop Hyssopus officinalis is on some pathogenic bacteria.
Methods: After collecting and confirming the scientific name, the methanolic extract of R. ribes L. and H. officinalis plants was prepared and the antimicrobial effects of the extracts by agar well diffusion and disk diffusion , as well as the determination of The minimum bactericidal concentration and the minimum inhibitory concentration (MIC / MBC) were dilution test on Staphylococcus aureus, Bacillus cereus, Escherichia coli and Pseudomonas aeruginosa.
Results: The highest growth inhibitory zone in S. aureus, B. cereus, P. aeruginosa, and E. coli at concentrations of 400 mg / ml, respectively, in the disc method of 13.21, 13.41, 11.2 and10.74 mm and the well method, respectively 13.64, 13.11, 10.67 mm, and 9.38 mm for the R. ribes L extract, and the disc method of 11.74, 10.2, 10.71, and 9.1 mm, and the well method of 12.41, 11.6, 10.2, and 9.9 respectively. 4.3 mm was observed for H. officinalis extract. The results of MBC / MIC showed that the extract of medicinal plants had the highest susceptibility to B. cereus bacteria and the least susceptibility to E. coli.
Conclusion: R. ribes L. and H. officinalis plants have significant inhibitory effects on the growth of pathogenic bacteria in vitro. Therefore, it can be expected that these extracts can be used for the treatment of bacterial infections and are a good alternative to the usual chemical treatments for the treatment of infections.
Methods: After collecting and confirming the scientific name, the methanolic extract of R. ribes L. and H. officinalis plants was prepared and the antimicrobial effects of the extracts by agar well diffusion and disk diffusion , as well as the determination of The minimum bactericidal concentration and the minimum inhibitory concentration (MIC / MBC) were dilution test on Staphylococcus aureus, Bacillus cereus, Escherichia coli and Pseudomonas aeruginosa.
Results: The highest growth inhibitory zone in S. aureus, B. cereus, P. aeruginosa, and E. coli at concentrations of 400 mg / ml, respectively, in the disc method of 13.21, 13.41, 11.2 and10.74 mm and the well method, respectively 13.64, 13.11, 10.67 mm, and 9.38 mm for the R. ribes L extract, and the disc method of 11.74, 10.2, 10.71, and 9.1 mm, and the well method of 12.41, 11.6, 10.2, and 9.9 respectively. 4.3 mm was observed for H. officinalis extract. The results of MBC / MIC showed that the extract of medicinal plants had the highest susceptibility to B. cereus bacteria and the least susceptibility to E. coli.
Conclusion: R. ribes L. and H. officinalis plants have significant inhibitory effects on the growth of pathogenic bacteria in vitro. Therefore, it can be expected that these extracts can be used for the treatment of bacterial infections and are a good alternative to the usual chemical treatments for the treatment of infections.
Type of Article: Original article |
Subject:
Basic Medical Sciences
Received: 2019/02/15 | Accepted: 2019/07/19 | Published: 2019/09/1
Received: 2019/02/15 | Accepted: 2019/07/19 | Published: 2019/09/1
References
1. Skaltsa H, Lazari DM, Chinou IB, Loukis AE.Composition and antibacterial activity of the essential oils of Stachys candida and S. chrysantha from Southern Greece. Planta Med. 1999;65 (3): 255- 256. [DOI] [Google Scholar]
2. Jafari-Sales A, Jafari B, Sayyahi J, Zohoori-Bonab T. Evaluation of antibacterial activity of ethanolic extract of malva neglecta and althaea officinalis l. On antibiotic-resistant strains of staphylococcus aureus. J Biol Today World. 2015;4(2):58-62. [DOI] [Google Scholar]
3. Mobaiyen H, Jafari Sales A, Sayyahi J. Evaluating antimicrobial effects of centaurea plant's essential oil on pathogenic bacteria: staphylococcus aureus, staphylococcus epidermidis, and escherichia coli isolated from clinical specimens. Journal of Fasa University of Medical Sciences. 2016 Mar 15;5(4):479-87. [Google Scholar]
4. Jafari-Sales A, Hossein-Nezhad P. Antimicrobial effects of Rosmarinus officinalis methanolic extract on Staphylococcus aureus, Bacillus cereus, Escherichia coli and Pseudomonas aeruginosa in laboratory conditions. Journal of Medicinal and Chemical Sciences. 2019 Sep 2:103-8. [Google Scholar]
5. Jafari-sales A, Shadi-Dizaji A. Evaluation of Inhibitory Effect of Methanol Extract of Allium Sativum in vitro on Staphylococcus aureus and Escherichia coli. Scientific Journal of Nursing, Midwifery and Paramedical Faculty. 2019 Jul 10;5(1):61-8 [Google Scholar]
6. WHO Traditional Medicine Strategy 2002-2005, Geneva. 2002;1-3:43-47.
7. Jafari-Sales A, Bolouri P. Evaluation of the antimicrobial effects of Glycyrrhiza glabra l. on some gram positive and gram negative pathogenic bacteria in laboratory conditions. Jorjani Biomed J. 2018; 6 (4) :78-84 [DOI] [Google Scholar]
8. Ozturk M, Ozturk FA, Duru ME, Topcu G. Antioxidant activ-ity of stem and root extracts of Rhubarb (Rheum ribes): An ed-ible medicinal plant. Food Chemistry. 2007; 103(2):623-30. doi: 10.1016/j.foodchem.2006.09.005 [DOI] [Google Scholar]
9. Onder Turkmen, Mustafa C rka, Suat E. Initial Evaluation of a New Edible Wild Rhubarb Species (Rheumribes L.) with a Modified Weighted Scaling Index Method. Pakistan Journal of Biological Sciences 2005; 8: 5, 763 - 5. [DOI] [Google Scholar]
10. zargari A. Rhubarbes. Medicinal plant. Volum 4: Sixt edition. Tehran University publication. 1997, pp: 234 - 9. 96Downloaded from jmp.ir at 15:25 +0430 on Tuesday May 21st 2019 [Google Scholar]
11. Qasemi dehkardy n. Rheumpalmatum L. Iranian herbal pharmacopoeia. 1st ed. Health Ministery press. Tehran Iran. 1381, pp: 342 - 9
12. Giasudin Jazaieri. Zaban Khorakiha. 1983, Volume 1, pp: 39 - 41
13. Gollan, W. (1992). Indian Vegetable Plantation. Akashdeep Publishing House. Delhi , India. [Google Scholar]
14. Ozcan MM, Dursun N, Arslan D. Some nutritional properties of Prangos ferulacea (L.) Lindl and Rheumribes L. stems growing wild in Turkey. Int. J. Food Sci. Nutr. 2007 58 (2): 162 - 7. [DOI] [Google Scholar]
15. Fatma TOSUN, Çiğdem AKYÜZ KIZILAY Anthraquinones and Flavonoids from Rheum ribes. Ankara Ecz. Fak. Derg. 2003; 32 (1): 31 - 5. [Google Scholar]
16. Bazzaz F, Khajehkaramadin M, Shokooheizadeh HR. In vitro antibacterial activity of Rheum ribes extract obtained from various plant parts against clinical isolates of Gram-negative pathogens. Iranian Journal of Pharmaceutical Research. 2010 Nov 20:87-91. [Google Scholar]
17. Khoshnood-Mansoorkhani MJ., Moein MR., Oveisi N. Anticonvulsant activity of Teucrium polium against seizure induced by PTZ and MES in mice. Iranian Journal of Pharmaceutical Research: IJPR2010; 9 (4): 395-401. PMCID: PMC3870063. [Google Scholar]
18. Azizi H., Keshavarzi M. Ethnobotanical study of medicinal plants of Sardasht , western Azerbaijan, Iran. Journal of Herbal Drugs (An International Journal on Medicinal Herbs)2015; 6 (2): 113-9. www.jhd.iaushk.ac.ir. [Google Scholar]
19. Ardestani A., Yazdanparast R. Inhibitory effects of ethyl acetate extract of Teucrium poliumon in vitro protein glycoxidation. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association2007; 45 (12): 2402-11. DOI:10.1016/j.fct.2007.06.020. [PubMed: 17673348]. [DOI] [Google Scholar]
20. Stojano vic G, Radulovic N, Hashimoto T, Palic R. In vitro antimicrobial activity of extracts of four Achillea species: The composition of Achillea clavennae L. (Asteraceae) extract. J Ethnopharmacol 2005; 101 (1 - 3): 185 - 10 [DOI] [Google Scholar]
21. Kim J, Kuk E, Nam Yu K, Kim JH, Park SJ, Lee HJ. Antimicrobial effects of silver nanoparticles. Nanomedicine: Nanotechnology, Biology, and edicine. 2007;3:95- 101. [DOI] [Google Scholar]
22. Sattari M, Shahbazi N, Najar Peeryeh S. An assessment of antibacterial effect of alcoholic and aquatic extracts of Eucalyptus leaves on Pseudomonas aeruginosa. J Med Sci. 2006;8(5):19-23 [Google Scholar]
23. Neef H, Declercq P, Laekeman G. Hypoglycaemic activity of selected European plants. Phytother Res 1995; 9:45-48. [DOI] [Google Scholar]
24. Shariff ZU. Modern herbal therapy for common ailments. Nature pharmacy series In Spectrum Books limited2001;1:9-84. [Google Scholar]
25. Alizadeh H, Jafari B, Babai T, The Study of Antibacterial Effect of Capsella BursaPastoris on Some of Gram Positive and Gram Negative Bacteria. Journal of Basic and Applied Scientific Research 2012; 2 (7): 6940 - 6945. [Google Scholar]
26. Dehghanzadeh, N., Ketabchi, S. and Alizadeh, A. 2012. Essential oil composition and antibacterial activity of Hyssopus officinalis L. grown in Iran. Asian Journal of Experimental Biological Science.3(4): 767- 771. [Google Scholar]
27. Hashemi, P., Abolghasemi, M., Fakhari, A., Ebrahimi, S. and Ahmadi, S. 2007. Hydrodistillation-solvent microextraction and GC-MS identification of volatile components of Artemisia aucheri . Chromatographia. 66: 283-286. [DOI] [Google Scholar]
28. Farzaneh, M., Ahmadzadeh, M., Hadian, J. and Tehrani, A.S. 2006. Chemical composition and antifungal activity of essential oils of three species of Artemisia on some soil borne phytopathogens. Communications in Agricultural and Applied Biological Sciences. 71(3): 1327- 1333. [Google Scholar]
29. Ghorbani-Ghouzhdi, H., Sahraroo, A., Asghari, H. and Abbasdokht, H. 2008. Composition of essential oils of Artemisia sieberi and Artemisia Khorasanica from Iran. World Applied sciences Journal. 5(3): 363-366. [Google Scholar]
30. Burt, S. 2004. Essential oils: Their antibacterial properties and potential applications in foods: A review. International Journal of Food Microbiology . 94(3): 223-253 [DOI] [Google Scholar]
31. Skandamis, P., Koutsoumanis, K., Fasseas, K.and Nychas, G.-J.E. 2001. Inhibition of oregano essential oil and EDTA on Escherichia coli O157:H7. Italian Journal of Food Science. 13(1): 65-75 [Google Scholar]
32. Soltani Nezhad, Sh., Mokhtari Sataeei, T. and Soltani Nezhad, M. 2010. Evaluation of antibacterial activity of eucalyptus leaf extract against Staphylococcus aureus, Escherichia coli and Streptococcus pyogenes in vitro, Journal of Islamic Azad University of Microbial Biotechnology. 4: 21-28.
33. HOVADIK, A.; CHLADEK, M., 1974: The antimicrobial activity of the essential oils of some aromatic plants. Bulletin, Vyzkumny Ustav Zelinarsky Olomouc (18): 61-71.
34. Hassanshahiyan, M., Saadatfar, A., Masoumi, F. Antimicrobial properties of Hyssopus officinalis extract against antibiotic-resistant bacteria in planktonic and biofilm form. Biological Journal of Microorganism, 2018; 7(28): 91-101. doi: 10.22108/bjm.2017.105971.1080 [Google Scholar]
35. Burfer, Jacob, Antibacterial activity of rhubarb hydroalcoholic extract on Staphylococcus aureus, Klebsiella pneumoniae and Escherichia coli, the first national congress of biology and natural sciences of Iran, 2014. [Google Scholar]
36. Salehi, A., Shariatifar, N., Salehi, A., Mohammadzadeh, A. In-Vitro Antimicrobial Effect of Aqueous and Alcoholic Extracts of Rheum Ribes on Some Food-Borne Pathogens. Journal of Sabzevar University of Medical Sciences, 2016; 23(3): 430-437. doi: 10.21859/sums-2303430 [DOI]
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