Jorjani Biomedicine Journal
فصلنامه علمی پژوهشی زیست پزشکی جرجانی
Jorjani Biomed J
Medical Sciences
http://goums.ac.ir/jorjanijournal
1
admin
2645-3509
10.52547/jorjanibiomedj
en
jalali
1397
6
1
gregorian
2018
9
1
6
3
online
1
fulltext
en
Evaluation of Different Proportions of Distilled Water to Substrate on Functional Properties, Antioxidant and Nutritional Quality of Bigeye Ilisha (Ilisha Megaloptera) protein hydrolysate
علوم پایه پزشکی
Basic Medical Sciences
تحقیقی
Original article
<strong>Background and Objectives:</strong> Production of fish protein hydrolysate is a method for converting the low-value economical underutilized fish species to value-added products. This study aimed to evaluate the different proportions of distilled water to the substrate on the functional characteristics, antioxidant and nutritional quality of fish protein hydrolysate of bigeye ilisha (<em>Ilisha megaloptera</em>) produced by enzymatic hydrolysis.<br>
<strong>Materials and Methods:</strong> After defatting of minced fish, the hydrolysis process was carried out using three different 4:1, 5:1 and 6:1 distilled water to substrate proportions by using alcalase enzyme in three replications. The protein hydrolysate samples were analyzed for approximate composition (soluble protein, moisture, fat and ash), functional characteristics (solubility, foam capacity, and foam stability), antioxidant properties (DPPH radical scavenging activity and reducing power) and mineral composition.<span dir="RTL"></span><br>
<strong>Results:</strong> In this study, the ratio of distilled water to the substrate affected the protein hydrolysate properties and the highest amounts of hydrolysis degree and DPPH radical scavenging activity were observed in samples with 5:1 ratio. Fish protein hydrolysate obtained from 4:1 ratio had the highest amount of soluble protein, and no significant difference was observed in term of solubility with samples obtained by 5:1 ratio. Moreover, there were no significant differences in terms of lightness and foam capacity of samples obtained from 4:1 and 6:1 proportions.<br>
<strong>Conclusion:</strong> According to the results of the study, using a 4:1 ratio of distilled water to substrate led to the production of fish protein hydrolysates from bigeye ilisha with higher functional properties and nutritional composition.
Alcalase Enzyme, Fish Protein Hydrolysate, Bigeye Ilisha (Ilisha Megaloptera), Functional Properties, Antioxidant Properties
24
39
http://goums.ac.ir/jorjanijournal/browse.php?a_code=A-10-206-18&slc_lang=en&sid=1
Aniseh
Jamshidi
10031947532846004330
10031947532846004330
No
PhD Student of Fish Processing, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
Bahareh
Shabanpour
b_shabanpour@yahoo.com
10031947532846004331
10031947532846004331
Yes
Department of Fisheries, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
Parastoo
Pourashouri
10031947532846004332
10031947532846004332
No
Assistant Professor of Department of Fisheries, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
Mojtaba
Raeisi
10031947532846004333
10031947532846004333
No
Cereal Health Research Center, Golestan University of Medical Sciences, Gorgan, Iran