Volume 9, Issue 3 (10-2021)                   Jorjani Biomed J 2021, 9(3): 13-23 | Back to browse issues page


XML Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

rezaei S M, Abedi B, Fatolahi H. The Simultaneous Effect of Linum Usitatissimum Supplementation and Aerobic Training on 6-Methylguanine and ATP in the Endothelial Aorta and Heart Tissues in Rats Poisoned with H2O2. Jorjani Biomed J 2021; 9 (3) :13-23
URL: http://goums.ac.ir/jorjanijournal/article-1-823-en.html
1- Ph.D. student, Department of Physical Education and Sport Sciences, Mahallat Branch, Islamic Azad University. Mahallat, Iran
2- Professor, Department of Physical Education and Sport Sciences, Mahallat Branch, Islamic Azad University. Mahallat, Iran , abedi@iaumahallat.ac.ir
3- Assistant Professor, Department of Physical Education, Pardis Branch, Islamic Azad University, Pardis, Iran
Abstract:   (3555 Views)
Background and Objective: As a high-energy demanding tissue, the heart is exposed to a high level of ROS molecules such as H2O2, leading to cardiovascular disorders through damaging macromolecules such as DNA and disrupting ATP production. Hence, this study aimed to investigate the simultaneous effect of aerobic exercise (Ae) and Linum Usitatissimum (Lu) supplementation on DNA damage and ATP synthesis in heart and aorta endothelial tissues in rats poisoned with hydrogen peroxide (H2O2).
Material and Methods: 56 male Wistar Albino rats were randomly divided into 7 groups, including HC (Healthy Control), TC (Toxicated Control), Lu1 (Received 5 mg/kg of Lu), Lu2 (Received 10 mg/kg of Lu), Ae (Received only Aerobic Exercise), Ae Lu1, and Ae Lu2. Then, all groups got poisoned by H2O2 except HC. Next, they received Linum Usitatissimum (Lu) supplementation and Low-Intensity Interval Training (LIIT). Finally, 24h after the last treatment session, the level of 6-methylguanine (6MG) and ATP were measured via the ELISA technique in cardiovascular tissue.
Results: The findings determined that Lu supplementation and Ae significantly diminish the 6-methyl guanine level in endothelial (F=111.3, p=0.0008, ƞ=0.9823) and heart cells (F=147.9, p=0.0005, ƞ=0.9867). Also, the ATP level was increased significantly in endothelial (F=342.6, p=0.0003, ƞ=0.9942) and heart cells (F=135.1, p=0.0013, ƞ=0.9854). However, no considerable changes were found for both factors in groups who received Ae or Lu singularly.
Conclusion: The study showed that concurrent administration of Lu and Ae could exert dynamic cardioprotective properties through their antioxidant effects.
Full-Text [PDF 881 kb]   (1323 Downloads) |   |   Full-Text (HTML)  (654 Views)  
Type of Article: Original article | Subject: Health
Received: 2021/04/18 | Accepted: 2021/07/25 | Published: 2021/09/29

References
1. Lee Y, Gustafsson ÅBJA. Role of apoptosis in cardiovascular disease. 2009; 14(4):536-48. [DOI] [PMID] [Google Scholar]
2. Favaloro B, Allocati N, Graziano V, Di Ilio C, De Laurenzi VJA. Role of apoptosis in disease. 2012; 4(5):330. [DOI] [PMID] [PMCID]
3. Csányi G. Oxidative stress in cardiovascular disease. Multidisciplinary Digital Publishing Institute; 2014. [view at publisher] [DOI] [PMID] [PMCID] [Google Scholar]
4. Xiao J, Sun B, Li M, Wu Y, Sun XBJJocp. A novel adipocytokine visfatin protects against H2O2‐induced myocardial apoptosis: a missing link between obesity and cardiovascular disease. 2013; 228(3):495-501. [view at publisher] [DOI] [PMID] [Google Scholar]
5. Chen K, Keaney JFJCar. Evolving concepts of oxidative stress and reactive oxygen species in cardiovascular disease. 2012; 14(5):476-83. [view at publisher] [DOI] [PMID] [PMCID] [Google Scholar]
6. Aldosari S, Awad M, Harrington EO, Sellke FW, Abid MRJA. Subcellular reactive oxygen species (ROS) in cardiovascular pathophysiology. 2018; 7(1):14. [view at publisher] [DOI] [PMID] [PMCID] [Google Scholar]
7. He F, Zuo LJIjoms. Redox roles of reactive oxygen species in cardiovascular diseases. 2015; 16(11):27770-80. [view at publisher] [DOI] [PMID] [PMCID] [Google Scholar]
8. Somyajit K, Gupta R, Sedlackova H, Neelsen KJ, Ochs F, Rask M-B, et al. Redox-sensitive alteration of replisome architecture safeguards genome integrity. 2017; 358(6364):797-802. [view at publisher] [DOI] [PMID] [Google Scholar]
9. Savina NV, Smal MP, Kuzhir TD, Egorova TM, Khurs OM, Polityko AD, et al. Biomarkers for genome instability in some genetic disorders: a pilot study. 2012;17(3):201-8. [view at publisher] [DOI] [PMID] [Google Scholar]
10. Papamichos-Chronakis M, Peterson CLJNRG. Chromatin and the genome integrity network. 2013; 14(1):62. [view at publisher] [DOI] [PMID] [PMCID] [Google Scholar]
11. Orth JD, Loewer A, Lahav G, Mitchison TJJMbotc. Prolonged mitotic arrest triggers partial activation of apoptosis, resulting in DNA damage and p53 induction. 2012; 23(4):567-76. [view at publisher] [DOI] [PMID] [PMCID] [Google Scholar]
12. Roos WP, Kaina BJCl. DNA damage-induced cell death: from specific DNA lesions to the DNA damage response and apoptosis. 2013; 332(2):237-48. [view at publisher] [DOI] [PMID] [Google Scholar]
13. Gomes EC, Silva AN, Oliveira MRdJOm, longevity c. Oxidants, antioxidants, and the beneficial roles of exercise-induced production of reactive species. 2012; 2012. [view at publisher] [DOI] [PMID] [PMCID] [Google Scholar]
14. Meirelles LRd, Matsuura C, Resende AdC, Salgado ÂA, Pereira NR, Coscarelli PG, et al. Chronic exercise leads to antiaggregant, antioxidant and anti-inflammatory effects in heart failure patients. 2014; 21(10):1225-32. [view at publisher] [DOI] [PMID] [Google Scholar]
15. Park S-Y, Kwak Y-SJJoer. Impact of aerobic and anaerobic exercise training on oxidative stress and antioxidant defense in athletes. 2016; 12(2):113. [DOI] [PMID] [PMCID] [Google Scholar]
16. Ashrafi J, Roshan VD, Mahjoub SJAJoP, Pharmacology. Cardioprotective effects of aerobic regular exercise against doxorubicin-induced oxidative stress in rat. 2012; 6(31):2380-8. [view at publisher] [DOI] [Google Scholar]
17. Shim YY, Gui B, Arnison PG, Wang Y, Reaney MJJTifs, technology. Flaxseed (Linum usitatissimum L.) bioactive compounds and peptide nomenclature: A review. 2014; 38(1):5-20. [view at publisher] [DOI] [Google Scholar]
18. Ramesh MJPiMS. Flax (Linum usitatissimum L.) fibre reinforced polymer composite materials: A review on preparation, properties and prospects. 2018. [view at publisher] [DOI] [Google Scholar]
19. Popa V-M, Gruia A, Raba D, Dumbrava D, Moldovan C, Bordean D, et al. Fatty acids composition and oil characteristics of linseed (Linum Usitatissimum L.) from Romania. 2012; 18(2):136-40.
20. Swanson D, Block R, Mousa SAJAin. Omega-3 fatty acids EPA and DHA: health benefits throughout life. 2012; 3(1):1-7. [view at publisher] [DOI] [PMID] [PMCID] [Google Scholar]
21. Tripathi V, Abidi A, Markerb S, Bilal SJIJPBS. Linseed and linseed oil: health benefits-a review. 2013; 3(3):434-42. [Google Scholar]
22. Herchi W, AMMAR KB, Bouali I, Abdallah IB, Guetet A, Boukhchina SJFS, et al. Heating effects on physicochemical characteristics and antioxidant activity of flaxseed hull oil (Linum usitatissimum L). 2016; 36(1):97-102. [view at publisher] [DOI] [Google Scholar]
23. Szewczyk M, Abarzua S, Schlichting A, Nebe B, Piechulla B, Briese V, et al. Effects of extracts from Linum usitatissimum on cell vitality, proliferation and cytotoxicity in human breast cancer cell lines. 2014; 8(5):237-45. [view at publisher] [DOI] [Google Scholar]
24. Tülüce Y, Özkol H, Koyuncu İJT, health i. Photoprotective effect of flax seed oil (Linum usitatissimum L.) against ultraviolet C-induced apoptosis and oxidative stress in rats. 2012; 28(2):99-107. [view at publisher] [DOI] [PMID] [Google Scholar]
25. Ghule AE, Jadhav SS, Bodhankar SLJAPJoTD. Effect of ethanolic extract of seeds of Linum usitatissimum (Linn.) in hyperglycaemia associated ROS production in PBMNCs and pancreatic tissue of alloxan induced diabetic rats. 2012; 2(5):405-10. [view at publisher] [DOI] [Google Scholar]
26. Nowsheen S, Yang ES. The intersection between DNA damage response and cell death pathways. Exp Oncol. 2012; 34(3):243-54. [view at publisher] [Google Scholar]
27. Garcia JJ, Martinez-Ballarin E, Robinson M, Allue JL, Reiter RJ, Osuna C, et al. Protective effect of beta-carbolines and other antioxidants on lipid peroxidation due to hydrogen peroxide in rat brain homogenates. Neurosci Lett. 2000; 294(1):1-4. [view at publisher] [DOI] [Google Scholar]
28. Kumar S, Srivastava N, Gomes J. The effect of lovastatin on oxidative stress and antioxidant enzymes in hydrogen peroxide intoxicated rat. Food Chem Toxicol. 2011; 49(4):898-902. [view at publisher] [DOI] [PMID] [Google Scholar]
29. Mohammed GJ, Hameed IHJIJoPHR, Development. Linum usitatissimum: Anti-bacterial activity, Chromatography, bioactive compounds, Applications: A review. 2018; 9(3):375-80. [view at publisher] [DOI] [Google Scholar]
30. Nikkhoi SK, Heydarzadeh H, Ranjbar S, Salimi F, Aghaeifard M, Alavian SM, et al. The Evaluation and Comparison of Transcriptionally Targeted Noxa and Puma Killer Genes to Initiate Apoptosis under Cancer-Specific Promoter CXCR1 in Hepatocarcinoma Gene Therapy. 2016; 16(10). [DOI] [PMID] [PMCID] [Google Scholar]
31. Takanlu JS, Mohammdi S, Ali SM, Rad H, Abroun S, Nikbakht MJCJ. Indirect Tumor Inhibitory Effects of MicroRNA-124 through Targeting EZH2 in The Multiple Myeloma Cell Line. 2020; 22(1). [view at publisher] [Google Scholar]
32. Abarzua S, Serikawa T, Szewczyk M, Richter D-U, Piechulla B, Briese VJAog, et al. Antiproliferative activity of lignans against the breast carcinoma cell lines MCF 7 and BT 20. 2012; 285(4):1145-51. [view at publisher] [DOI] [PMID] [Google Scholar]
33. Ranjbar R, Karimian A, Aghaie Fard A, Tourani M, Majidinia M, Jadidi‐Niaragh F, et al. The importance of miRNAs and epigenetics in acute lymphoblastic leukemia prognosis. 2019; 234(4):3216-30. [view at publisher] [DOI] [PMID] [Google Scholar]
34. Vásquez‐Trincado C, García‐Carvajal I, Pennanen C, Parra V, Hill JA, Rothermel BA, et al. Mitochondrial dynamics, mitophagy and cardiovascular disease. 2016; 594(3):509-25. [view at publisher] [DOI] [PMID] [PMCID] [Google Scholar]
35. Van Houten B, Hunter SE, Meyer JNJFib. Mitochondrial DNA damage induced autophagy, cell death, and disease. 2016; 21:42. [view at publisher] [DOI] [PMID] [Google Scholar]
36. Jin B, Robertson KD. DNA methyltransferases, DNA damage repair, and cancer. Epigenetic Alterations in Oncogenesis: Springer; 2013. p. 3-29. [view at publisher] [DOI] [PMID] [PMCID] [Google Scholar]
37. Velalopoulou A, Tyagi S, Pietrofesa R, Arguiri E, Christofidou-Solomidou MJIjoms. The flaxseed-derived lignan phenolic secoisolariciresinol diglucoside (SDG) protects non-malignant lung cells from radiation damage. 2016; 17(1):7. [view at publisher] [DOI] [PMID] [PMCID] [Google Scholar]
38. Moree SS, Rajesha JJM, biochemistry c. Investigation of in vitro and in vivo antioxidant potential of secoisolariciresinol diglucoside. 2013; 373(1-2):179-87. [view at publisher] [DOI] [PMID] [Google Scholar]
39. Liu C-M, Zheng G-H, Ming Q-L, Chao C, Sun J-MJJoa, chemistry f. Sesamin protects mouse liver against nickel-induced oxidative DNA damage and apoptosis by the PI3K-Akt pathway. 2013; 61(5):1146-54. [view at publisher] [DOI] [PMID] [Google Scholar]
40. Harper A, Kerr DJ, Gescher A, Chipman JKJFRR. Antioxidant effects of isoflavonoids and lignans, and protection against DNA oxidation. 1999; 31(2):149-60. [view at publisher] [DOI] [PMID] [Google Scholar]
41. Ghule AE, Jadhav SS, Bodhankar SLJIi. Trigonelline ameliorates diabetic hypertensive nephropathy by suppression of oxidative stress in kidney and reduction in renal cell apoptosis and fibrosis in streptozotocin induced neonatal diabetic (nSTZ) rats. 2012; 14(4):740-8. [view at publisher] [DOI] [PMID] [Google Scholar]
42. Zhao G, Etherton TD, Martin KR, Gillies PJ, West SG, Kris-Etherton PMJTAjocn. Dietary α-linolenic acid inhibits proinflammatory cytokine production by peripheral blood mononuclear cells in hypercholesterolemic subjects. 2007; 85(2):385-91. [view at publisher] [DOI] [PMID] [Google Scholar]

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Jorjani Biomedicine Journal

Designed & Developed by : Yektaweb