Medical Nanotechnology and Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran , khoshnevisan.k@sbmu.ac.ir
Abstract: (1533 Views)
Serotonin (5-hydroxytryptamine, 5-HT) is a pivotal neurotransmitter involved in a myriad of physiological processes, including the regulation of mood, appetite, and sleep cycles. Accurate quantification of 5-HT levels in human serum is fundamental to elucidating its pathophysiology in various clinical conditions and disorders. Over the recent years, electrochemical sensing techniques have emerged as highly reliable and sensitive modalities for 5-HT detection. This article details a protocol study designed to determine 5-HT concentrations in human serum using a validated electrochemical sensing platform. The protocol encompasses a comprehensive overview of electrochemical sensing techniques, methodology validation, sample collection and processing, experimental procedures for 5-HT determination, and subsequent data analysis. The outcomes of this study hold significant potential for advancing the current understanding of 5-HT-associated disorders and may facilitate the development of novel, targeted therapeutic interventions.
References
1. Nimgampalle M, Chakravarthy H, Sharma S, Shree SH, Bhat AR, Pradeepkiran JA,et al. Neurotransmitter systems in the etiology of major neurological disorders: Emerging insights and therapeutic implications. Ageing Res Rev. 2023:89:101994. [View at Publisher] [DOI] [PMID] [Google Scholar]
2. Kanova M, Kohout P. Serotonin-Its Synthesis and Roles in the Healthy and the Critically Ill. Int J Mol Sci. 2021;22(9):4837. [View at Publisher] [DOI] [PMID] [Google Scholar]
3. Khoshnevisan K, Chehrehgosha M, Sajjadi-Jazi SM, Meftah AM. Tryptophan and serotonin levels as potent biomarkers in diabetes mellitus complications: a new approach of diagnostic role. J Diabetes Metab Disord. 2022;21(2):1923-34. [View at Publisher] [DOI] [PMID] [Google Scholar]
4. Khoshnevisan K, Honarvarfard E, Torabi F, Maleki H, Baharifar H, Faridbod F, et al. Electrochemical detection of serotonin: A new approach. Clin Chim Acta. 2020:501:112-119. [View at Publisher] [DOI] [PMID] [Google Scholar]
5. Khoshnevisan K, Maleki H, Honarvarfard E, Baharifar H, Gholami M, Faridbod F, et al. Nanomaterial based electrochemical sensing of the biomarker serotonin: a comprehensive review,. Microchim Acta. 2019;186(1):49. [View at Publisher] [DOI] [PMID] [Google Scholar]
6. Jones AW .Brief history of the alcohol biomarkers CDT, EtG, EtS, 5-HTOL, and PEth. Drug Test Anal. 2024;16(6):570-87. [View at Publisher] [DOI] [PMID] [Google Scholar]
7. Guillén-Casla V, Rosales-Conrado N, León-González ME, Pérez-Arribas LV, Polo-Díez LM. Determination of serotonin and its precursors in chocolate samples by capillary liquid chromatography with mass spectrometry detection. J Chromatogr A. 2012:1232:158-65. [View at Publisher] [DOI] [PMID] [Google Scholar]
8. Chapin AA, Han J, Ghodssi R. Adsorption Kinetic Model Predicts and Improves Reliability of Electrochemical Serotonin Detection. Methods Protoc. 2023;6(1):6. [View at Publisher] [DOI] [PMID] [Google Scholar]
9. Khoshnevisan K, Baharifar H, Torabi F, Sadeghi Afjeh M, Maleki H, Honarvarfard E, et al. Serotonin level as a potent diabetes biomarker based on electrochemical sensing: a new approach in a zebra fish model. Anal Bioanal Chem. 2021;413(6):1615-27. [View at Publisher] [DOI] [PMID] [Google Scholar]
10. Rivkin B, Fassl P, Sun Q, Taylor AD, Chen Z, Vaynzof Y. Effect of Ion Migration-Induced Electrode Degradation on the Operational Stability of Perovskite Solar Cells. ACS Omega. 2018;3(8):10042-7. [View at Publisher] [DOI] [PMID] [Google Scholar]
11. Azzouz A, Ballesteros E. Gas chromatography-mass spectrometry determination of pharmacologically active substances in urine and blood samples by use of a continuous solid-phase extraction system and microwave-assisted derivatization. J Chromatogr B Analyt Technol Biomed Life Sci. 2012:891-892:12-9. [View at Publisher] [DOI] [PMID] [Google Scholar]
12. Zhou J, Sheng M, Jiang X, Wu G, Gao F. Simultaneous Determination of Dopamine, Serotonin and Ascorbic Acid at a Glassy Carbon Electrode Modified with Carbon-Spheres. Sensors (Basel). 2013;13(10):14029-40. [View at Publisher] [DOI] [PMID] [Google Scholar]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
