Golestan University of Medical Sciences
Jorjani Biomedicine Journal
2645-3509
6
1
2018
3
1
DNA hypomethylation of the nuclear receptor subfamily 5 (NR5A1) gene promoter is associated with endometriosis among women in north west of Iran
1
11
EN
Soraya
Larki
MSc of Genetic, Department of Biology, Islamic Azad University, Tabriz Branch, Tabriz, Iran
Masoud
Maleki
Assistant Professor, Department of Biology, Islamic Azad University, Tabriz Branch, Tabriz, Iran
Background and objectives: Endometrial tissue growth and its activity outside the uterus cause endometriosis. It has been suggested that various epigenetic deviations play a major role in the pathogenesis of endometriosis. Steroidogenic factor 1 (SF-1; NR5A1) is an essential transcription factor for estrogen biosynthesis in endometrial cells. The expression of SF-1 in endometriosis and lack of expression in normal endometrium is primarily determined by its promoter methylation. Here, we aimed to compare the methylation status of the SF-1 gene promoter region in women with endometriosis in comparison to healthy subjects.
Methods: In the present case–control study, DNA was extracted from 25 endometrial tissue samples from women with endometriosis and 5 normal post-hysterectomy endometrium tissues which were collected from Tabriz hospitals including Vali-e-Asr, Taleghani, 29 Bahman and Shams in 2016. The obtained DNA samples were subjected to Bisulfite-treatment. Finally, the status of SF-1gene promoter methylation was evaluated by methylation specific PCR method. Statistical analyses including descriptive and inferential statistics were conducted using tables, bar charts by statistical software SPSS version 20 and independence test.
Results: The methylation status of SF-1 gene promoter was decreased significantly in endometriosis samples (P<0.05).
Conclusion: SF-1 gene promoter hypomethylation could increase the relative expression of SF-1 gene in endometriosis which may lead to the development or progression of the disease.
Golestan University of Medical Sciences
Jorjani Biomedicine Journal
2645-3509
6
1
2018
3
1
Healing effects of Ziziphus jujuba hydroalcoholic extract with exercise training on histopathological changes of male wistar rats testicular tissue in response to Boldenone steroid administration
12
21
FA
Behrooz
Yahyaei
Department of Basic Science, Faculty of Medical Sciences, Shahrood Branch, Islamic Azad University, Shahrood, Iran
Mahnaz
Nouri
Department of Obstetrics and Gynecology, Faculty of Medical Sciences, Shahrood Branch, Islamic Azad University, Shahrood, Iran
Hamid
Matmir
Department of Obstetrics and Gynecology, Faculty of Medical Sciences, Shahrood Branch, Islamic Azad University, Shahrood, Iran
Background and objectives: Anabolic steroids are routinely consumed by athletes. Therefore, this study was conducted to investigate the effect of Boldenone (BOL) on testicular tissue and the healing effect of jujube (Ziziphus jujuba) hydroalcoholic extract in addition to physical exercise.
Methods: In the present case–control study, 42 male wistar rats were purchased and divided into 3 experimental groups. 28 rats were subjected to BOL injection and subsequently divided into 4 groups. The control group, Sham and 8-week BOL group which was also divided into 4 subsequent groups of controlling BOL complications after 8 weeks, untreated control, jujube extract and jujube extract along with physical exercise.
Results: In the present study we found that jujube extract exerted healing effects on all groups pf treated rats in addition to the exercise training groups.
Conclusion: Jujube extract along with physical exercise may exert healing effects on testicular tissue after administration of anabolic BOL steroid.
Golestan University of Medical Sciences
Jorjani Biomedicine Journal
2645-3509
6
1
2018
3
1
Evaluation of the Bactericidal Effects of Zingiber officinale, Aloysia citrodora and Artemisia dracunculus on the Survival of Standard Gram-Positive and Gram-Negative Bacterial Strains
22
32
FA
Mahdi
Tajbakhsh
Bachelor of Plant Biology, Department of Life Sciences, Shahid Beheshti University, Tehran, Iran
Neda
Soleimani
Assistant Professor, Medical Microbiology, Department of Microbiology and Microbial Biotechnology, Faculty of Life Science and Biotechnology, Shahid Beheshti University, Tehran, Iran
Background and objectives: Historically, herbs and plants have been used for their therapeutic properties in the form of flavors and preservatives. Recently, the application of medicinal herbs has increased considering their numerous benefits and minimum side-effects. Treatment of bacterial infections is currently a major challenge in the healthcare systems across the world. The present study aimed to assess the bacterial effects of Zingiber officinale, Aloysia citrodora and Artemisia dracunculus essential oils on the survival of standard Gram-positive and Gram-negative bacterial strains.
Methods: In the present experimental study, we evaluated the effects of Tarragon (A. dracunculus), Ginger (Z. officinale) and Lemon Beebrush (A. citrodora) essential oils on 6 strains of Staphylococcus aureus, Klebsiella spp, Salmonella typhimurium, Staphylococcus epidermidis, Proteus spp and Corynebacterium diphtheriae. The well-diffusion method was applied to assess the antibacterial properties of the essential oils. Moreover, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) tests were used to determine the bacterial and inhibitory concentrations of the extracts.
Results: MIC and MBC results demonstrated that the ginger extract (0.125 mg/mL) had the most significant impact on Staphylococcus aureus, Salmonella typhimurium, and Staphylococcus epidermidis. Furthermore, tarragon extract (0.03125 mg/mL) had the most significant effect on Staphylococcus aureus, Proteus spp, and Corynebacterium diphtheriae. The antibacterial effects of these essential oils were not observed on other bacteria.
Conclusion: Medicinal plants have long been used for their therapeutic properties. According to the results, ginger and tarragon extracts are effective combinations for the treatment of the infections caused by Gram-negative and Gram-positive bacteria.
Golestan University of Medical Sciences
Jorjani Biomedicine Journal
2645-3509
6
1
2018
3
1
Association of Socioeconomic Status and Pro-Environmental Behaviors in the Citizens of Gorgan, Iran (2017)
33
43
FA
Danial
Bagheri
PhD Candidate in Sociology, Islamic Azad University, Babol Branch, Babol, Iran
Reza Ali
Mohseni
Associate Professor, School of Humanities, Islamic Azad University, Central Tehran Branch, School of Humanities, Islamic Azad University, Babol Branch, Babol, Iran
Seyed Mohammad Sadegh
Mahdavi
Professor, Shahid Beheshti University, School of Humanities, Islamic Azad University, Babol Branch, Babol, Iran
Background and objectives: Environmental pollution is a major cause of various diseases. Massive production of hospital, industrial, and household wastes lead to several health issues, threatening community health on a daily basis. The present study aimed to determine the association between socioeconomic status and pro-environmental behaviors in the citizens of Gorgan, Iran.
Methods: This cross-sectional, descriptive-analytical study was conducted on 400 citizens of Gorgan. Participants were divided into three regions based on urban categorization. Data were collected using demographic and socioeconomic questionnaires. To assess environmental behaviors, a standard questionnaire was used based on the Dunlap spectrum. Data analysis was performed in SPSS version 16 using the analysis of variance (ANOVA), independent samples t-test and factor analysis.
Results: No positive significant correlation was observed between gender and environmental behaviors. The results of ANOVA showed a significant association between marital status and environmental behaviors. In addition, the results of factor analysis indicated that five factors explained 55.49% of environmental behaviors. The results of ANOVA also demonstrated that middle-class citizens had a more responsible attitude toward the environment compared to others (P<0.05).
Conclusion: According to the results, socioeconomic status influenced the social value orientations and responsible behaviors of individuals toward the environment. Therefore, increasing the quality of life and providing comprehensive education could enhance pro-environmental behaviors and promote community health.
Golestan University of Medical Sciences
Jorjani Biomedicine Journal
2645-3509
6
1
2018
3
1
The Effectiveness of Cognitive-Behavioral Therapy on Emotional Control of MS Patients in the City of Isfahan
44
54
FA
Maedeh Sadat
Alavi
Department of Psychology, Najaf Abad Branch, Islamic Azad University, Najaf Abad, Iran
Sheida
Jabal Ameli
Department of Psychology, Najaf Abad Branch, Islamic Azad University, Najaf Abad, Iran
Background and objectives: Cognitive-behavioral therapy is one of the interventions used in the treatment of chronic medical conditions such as MS in recent years. Hence, this study was designed to determine the effectiveness of cognitive-behavioral therapy on emotional control of MS patients in the city of Isfahan.
Methods: The present study was a semi-experimental research which was conducted by using a pretest-posttest design with the control group. The study population consisted of all members of the MS Society of Isfahan in 2016-2017. Using the available sampling method, 30 subjects were selected and randomly divided into two experimental and control groups (15 subjects for each group). Both groups completed Williams and Chambless emotional control questionnaire as the pre-test. After eight sessions (two one-hour sessions per week for four weeks) of cognitive-behavioral therapy, the posttest was performed for the groups. The data were analyzed using descriptive statistical methods, including mean and standard deviation and inferential statistics including univariate covariance analysis in the SPSS 20 software.
Results: The study participants included 16 married women and 14 married men. The mean age of the participants in the experimental and control groups was 30.23 ± 7.06 and 29.34 ± 7.15, respectively. The results of the univariate covariance analysis showed that a significant difference in the mean scores of emotional control from the pre-test to the post-test stage between the experimental group and the control group (P = 0.001). Thus, in the experimental group, the mean total score of emotional control in the pretest and post-test phases was equal to 71.66 and 31.33, respectively, while these values in the control group in the pre-test and post-test phases were 67.06 and 62.93, respectively. Also in the experimental group, the scores of four sub-scales of anger, depressed mood, anxiety, and positive emotions revealed significant differences between the post-test stage and the pre-test stage (P =0.001). Therefore, the cognitive-behavioral therapy was effective in improving the emotional control of MS patients.
Conclusion: Considering the effectiveness of cognitive-behavioral therapy, we recommended the use of this therapeutic method to improve the emotional control of the patients with MS.
Golestan University of Medical Sciences
Jorjani Biomedicine Journal
2645-3509
6
1
2018
3
1
Association between Homesickness and the Related Factors with Happiness in the Dormitory Students of Hamadan University of Medical Sciences, Iran
55
66
EN
Babak
Moeini
Associate Professor of Health Education, Social Determinants of Health Research Center, Hamedan University of Medical Sciences, Hamedan, Iran
Hamid
Abbasi
PhD Candidate in Health Education and Promotion, Student Research Center, Hamedan University of Medical Sciences, Hamedan, Iran
Maryam
Afshari
PhD Candidate in Health Education and Promotion, Student Research Center, Hamedan University of Medical Sciences, Hamedan, Iran
Morteza
Haji Hosseini
MSc in Biostatistics, Center for Atherosclerosis and Coronary Artery Research, Birjand University of Medical Sciences, Birjand, Iran
Soheila
Rashidi
MSc Student in Health Education, Student Research Center, Hamedan University of Medical Sciences, Hamedan, Iran
Background and objectives: Admission to university is an important event in the life of youth in every country. Homesickness is one of the most significant issues faced by university students during their education life. The present study aimed to determine the association between homesickness, happiness and the related factors in the dormitory students in Hamadan University of Medical Sciences, Iran.
Methods: This cross-sectional, descriptive-analytical study was conducted on 387 dormitory students at Hamedan University of Medical Sciences in Hamedan, Iran during February-March 2015. Subjects were selected via stratified random sampling with proportional allocation. Data were collected using self-report and using demographic and contextual questionnaires, Fenfelit homesickness questionnaire, and oxford happiness questionnaire. Data analysis was performed in SPSS version 23 using Pearson’s correlation-coefficient and general linear model (GLM) at the significance level of 0.05.
Results: The mean score of homesickness in both genders had a significant difference (P=0.009). In addition, the mean score of happiness differed in terms of economic status. Homesickness was negatively correlated with the happiness score, so that increased homesickness was associated with reduced happiness. Variables of homesickness, age, internet use, maternal education, health status, economic status, and education level could significantly predict happiness.
Conclusion: Mental health of students in dormitories is of paramount importance. Therefore, interventions and preventive programs must recognize the causes of happiness, and appropriate interventions should be developed and implemented based on the influential factors in this regard.
Golestan University of Medical Sciences
Jorjani Biomedicine Journal
2645-3509
6
1
2018
3
1
Epidemiological Study of the Suburban Accident Mortalities Recorded in Golestan, Iran in 2015
67
73
EN
Farzaneh
Afkhaminia
Student in Biostatistics, Student Research Committee, School of Health, Mazandaran University of Medical Sciences, Sari, Iran
Jamshid
Yazdani Charati
Associate Professor, Department of Biostatistics, School of Health, Mazandaran University of Medical Sciences, Sari, Iran
Elaheh
Rahimi
Student in Biostatistics, Student Research Committee, School of Health, Mazandaran University of Medical Sciences, Sari, Iran
Nourodin
Mousavi Nasab
Associate Professor, Department of Biostatistics, School of Health, Mazandaran University of Medical Sciences, Sari, Iran
Background and objectives: Road accidents are one of the most important causes of mortality and severe physical and psychological damage which may lead to adverse social, cultural and economic consequences in the human community. Frequency and severity of road accidents in developing countries are noticeably higher in comparison to developed countries. In Iran, 25% of casualties are due to the abnormal deaths caused by road accidents. It is estimated that more than 22,000 people die due to road accidents every year. The present study aimed to epidemiologically investigate the mortality rate of suburban accidents in Golestan province, Iran.
Methods: This cross-sectional study was conducted using a descriptive approach. Required data were obtained from the traffic police of Golestan province. In total, 2,922 cases of road accidents were investigated in Golestan province in 2015. The analyzed data included the demographic characteristics of the deceased and the environmental and geographical conditions of the accident. Data analysis was performed in SPSS version 20.
Results: Among 2,922 road accidents in Golestan province, 251 cases led to the death of 317 individuals. Most of the accidents leading to death were by automobiles (69.7%) and due to distraction from the road (33.1%), which occurred on main roads (47.8%). Moreover, 29.7% of guilty drivers had not fastened seatbelts. Motorcycle riders and car passengers accounted for the highest percentage of accident victims (30.6% and 25.5%, respectively).
Conclusion: Education and emphasis on the use of seatbelts and motorcycle helmets while driving seem essential to reducing the injuries caused by road traffic accidents. Considering that most road accidents occur due to the distraction of the driver from the road, changing traffic behaviors to improve discipline is of paramount importance.